MCLAB/T7 RNA Polymerase/RPOEM/Any Size

Description:
T7RNAPolymeraseisaDNA-dependentRNApolymerasederivedfromtheT7bacteriophagewhichexhibitsahighrecognitionspecificitytotheT7promoterandterminatorsequencesandcatalyzesthe5'->3'synthesisofRNAstartingataT7promotersequence^(1,2).

SpecificActivity:312,500U/mg

Application:
-RadiolabeledRNAprobepreparation
-RNAgenerationforinvitrotranslation
-RNAgenerationforstudiesofRNAstructure,processingandcatalysis
-Expressioncontrolviaanti-senseRNA

Source:
PurifiedfromastrainofE.colithatexpressestherecombinantT7RNAPolymerasegene.

Suppliedin:
50mMTris-HCl
100mMNaCl
1mMDTT
1mMEDTA
50%Glycerol
0.1%TritonX-100
pH7.9@25°C

Suppliedwith:
10xT7RNAPolymeraseBuffer
400mMTris-HCl
60mMMgCl₂
100mMDTT
20mMSpermidine
pH7.9@25°C

UnitDefinition:
Oneunitisdefinedastheamountofenzymethatwillincorporate1nmolofATPintoacid-precipitablematerialin1hourat37°C.

RecommendedStorageCondition:-20°C

References:
1.Chamberlin,M,etal.(1973)J.Biol.Chem.248,2235-2244,2245-2250.
2.Chamberlin,M.etal.(1982)inTheEnzymes,3rdedition,ed.P.D.Boyer(AcademicPress,NewYork.)15,87-108.