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MCLAB/Inorganic Pyrophosphatase, E. coli/IPE100/0.25 mg
Inorganicpyrophosphatase(PPase)isubiquitousinnatureandplaysanimportantroleinenergymetabolism,providingathermodynamicpullforbiosyntheticreactionssuchasprotein,RNA,andDNAsynthesis.

Description:
Inorganicpyrophosphatase(PPase)isubiquitousinnatureandplaysanimportantroleinenergymetabolism,providingathermodynamicpullforbiosyntheticreactionssuchasprotein,RNA,andDNAsynthesis.EscherichiacoliK-12geneppaencodinginorganicpyrophosphatase(PPase)wasclonedandsequenced.The5'-endoftheppamRNAwasidentifiedbyprimerextensionmapping.

Source:
E.colistraincarryingaplasmidencodingpyrophosphatasefromE.coliK-12.

Application:
-Roleinprotein,RNA,andDNAsynthesis
-CatalyzingthereactionPPi+H2O—>2Pi

UnitDefinition:
Oneunitwillrelease1.0µmoleofinorganicorthophosphateperminuteatpH8.5at37°C.

SpecificActivity: 3,500U/mg

RecommendedStorageCondition:-20°C

Reference:
Kornberg,A.1962.Onthemetabolicsignificanceofphosphorolyticandpyrophosphorolyticreactions,p.251-264.InH.KashaandP.Pullman(ed.),Horizonsinbiochemistry.AcademicPress,NewYork.


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