MCLAB/HoTaq DNA Polymerase (hot start)/HT205/2,500 Units,5 U/μl

Description:
HoTaq™DNAPolymeraseisahot-startTaqDNAPolymerase,whichisachemically modifiedformofTaqDNAPolymerase.HoTaqDNAPolymeraseisprovidedinaninactivestateandhasaminimumenzymaticactivityatambienttemperatures.Itwillbecomeactiveafter10minutesheatingat95ºC.Thispreventstheformationofmisprimedproductsduringreactionsetupandthefirstdenaturationstep,leadstohighPCRspecificity.Itissuitablefordiagnosticreactionwithouttheminerband.Theenzymeisahighprocessive5'->3'DNApolymerasethatlacks3'->5'exonucleaseactivity.EachlotofHoTaqDNApolymeraseistestedforPCRamplification.

Suppliedwith:
10xTaqPCRBuffer(NodNTP)

Suppliedin:
20mMTris-HCl(pH8.0)
100mMKCl
0.5%Tween20
0.1mMEDTA
1mMDTT
50%(v/v)glycerol

Comparison:
HereistheresultofcomparingMCLAB'sHoTaqwithotherleadingbrands.



Source:
AnE.colistrainthatcarriestheTaqDNAPolymerasegenefromThermusaquaticus(sameasTaqDNAPolymerase).


RecommendedStorageCondition:-20°C

RecommendedReactionConditions:
95°C,10minutes.->(95°C,10seconds.->55°C,30seconds.->72°C,30seconds.)for25cycles.

UnitDefinition:
Oneunitisdefinedastheamountofenzymethatwillincorporate10nmolofdNTPsintoacid-insolublematerialin30minutesat75°C.

References:
1.ChienA,EdgarDB,TrelaJM(1976)."DeoxyribonucleicacidpolymerasefromtheextremethermophileThermusaquaticus".J.Bact.127(3):1550–7.PMC232952.PMID8432.
2.Saiki,RK;etal.(1988)."Primer-directedenzymaticamplificationofDNAwithathermostableDNApolymerase.".Science239(4839):487–91.doi:10.1126/science.2448875.PMID2448875.
3.Saiki,RK;etal.(1985)."Enzymaticamplificationofbeta-globingenomicsequencesandrestrictionsiteanalysisfordiagnosisofsicklecellanemia".Science230(4732):1350–4.doi:10.1126/science.2999980.PMID2999980.
3.LawyerFC,etal.(1993)."High-levelexpression,purification,andenzymaticcharacterizationoffull-lengthThermusaquaticusDNApolymerase...".PCRMethodsAppl.2(4):275–87.PMID8324500.