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MCLAB/Dh10Beta/DH10100/1 Ea188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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MCLAB/Dh10Beta/DH10100/1 Ea
TheDH10BcompetentE.colihasatransformationefficiencyof1x109cfu/µgforsupercoiledDNAandisidealforhigh-efficiencycloningandplasmidpropagation.Itallowsstablereplicationofhigh-copynumberplasmids.IncomparisonwithDH5a,methylatedDNAfromgenomicpreparation couldbeefficientlytransformedintoDH10B,therefor,theDH10BcellsaremoreapplicablethanDH5aingeneratinggenomiclibrariescontainingmethylatedcytosineoradenineresidues.

Description:
TheDH10BcompetentE.colihasatransformationefficiencyof1x109cfu/µgforsupercoiledDNAandisidealforhigh-efficiencycloningandplasmidpropagation.Itenablesstablereplicationsofhigh-copynumberplasmids.IncomparisonwithDH5a,methylatedDNAfromgenomicpreparation couldbeefficientlytransformedintoDH10B,thereforDH10BcellsaremoreapplicablethanDH5aingeneratinggenomiclibrariescontainingmethylatedcytosineoradenineresidues.

Application:

  • Highesttransformationefficiency
  • Generalcloning
  • Blue-whiteselection
  • ReducedrecombinationofclonedDNA(recA1)

Recommendedstoragecondition:
Thisproductshouldbestoredat-80°C.Thawoniceonlybeforeuse.Donotre-freeze.

Genotype:
F-mcrAΔ(mrr-hsdRMS-mcrBC)Φ80dlacZΔM15ΔlacX74endA1recA1deoRΔ(ara,leu)7697araD139galUgalKnupGrpsLλ--

 CompetentCellType:
Chemically

TransformationEfficiency(cfu/μg):
>1x109

Blue-WhietScreening:
Yes

Strain:
K12

ReducesRecombination:
Yes

CloningMethylatedDNA:
Yes

ImprovesPlasmidQuality:
Yes

PreparingUnmethylatedDNA:
Notsuitable

T1PhageResistant:
No

RecADeficien:
Yes


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