MCLAB/T4 Lysozyme/T4LY100/1 mg

Description:
BacteriophageT4Lysozymebreaksdownbacterialcellwalls.Theenzymeattacksthepeptidoglycansinthecellwallsofbacteriaandhydrolyzestheß-1,4linkagesbetweenN-acetylmuramicacidandN-acetylglucosamine.SpecificactivityofT4lysozymeissignificantlygreaterthaneggwhitelysozymewhenassayedwithMicrococcuslysodeikticusandE.coli.

Application:
-Bacteriallysisfornucleicacidextraction
-Bacteriallysisforrecombinantproteinextraction

Source:
ArecombinantE.colistraincarryingtheclonedbacteriophageT4lysozymegene.

UnitDefinition:
OneunitofT4lysozymeproducesadecreaseinA₃₅₀of0.008persecondat23°Cwitha0.5mg/mlsuspensionoflyophilizedM.lysodeikticusin0.02Mphosphatebuffer(pH6.7).

RecommendedStorageCondition:-20°C

Reference:
Tsugita,A.,andInouye,M.PurificationofBacteriophageT4Lysozyme.TheJournalofBiologicalChemistry,243,391-397.(1968)