MCLAB/2X HotSybr qPCR Kit/HSM400/1 Ea

ForResearchUseOnly.

Figure:AmplificationofhumanGAPDHgenetargetwith2XHotSybrReal-timePCRKit.Amplificationcurvesareshownfortenfolddilutionsof0.0002pMto20pMofplasmid.Insetsshowthedissociationprofileandthestandardcurvedata.

Description:
Thisisahighperformancereal-timePCRreagent.ItutilizesMCLAB'sproprietaryquantitativePCRtechnology.

Advantages:
2xHotSybrPCRReactionMixproductscutthetotalreactiontimedowntohalf.


-Normallythetotalreactiontimeis4350seconds:95°C,10minutes=>(95°C,15seconds=>60°C,60seconds)x50
-ForMCLAB's2xHotSybrPCRReactionMix,thetotalreactiontimeisreducedto2350seconds:95°C,10minutes=>(95°C,5seconds=>60°C,30seconds)x50

Application:
SybrgreendyebasedquantitativePCR:includingDNAquantification,2-stepRTPCR,SNPanalysis,etc.

Primerandprobedesign:
Appropriatesoftware,suchasABIPrimerExpress^TM,shouldbeused.

RecommendedReactionConditions:
95°C,10minutes->(95°C,5seconds.->60°C,30seconds.)for50cycles->meltingcurve.

RecommendedStorageConditions:-20°C

Notes:
Toachieveaccuratequantification,itishighlyrecommendedto(1)doreplicates;(2)reducepipettingerror;(3)primerconcentrationfrom100nMto300nM;(4)runmeltingcurvefollowingamplificationcycles.

Reference:
HiguchiR,DollingerG,WalshPS,GriffithR;Bio/Technology10:413-417,1992