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MCLAB/5’ DNA Adenylation Kit/APPK100/1 Ea

5´DNAAdenylationKitisfortheenzymaticsynthesisof5’adenylatedssDNAlinkers.

Description:
5´DNAAdenylationKitisfortheenzymaticsynthesisof5adenylatedssDNAlinkers.ThekitisoptimizedtoproducetheadenylatedDNAwithorwithout3´terminator.The5´DNAadenylationkitcansimplyandefficientlygenerategreaterthan95%conversionofpDNAtoAppDNA.

Features:

  • Onestepreaction.Simpleandefficient.
  • 65°Creactiontemperaturereducessecondarystructuralconcerns.
  • Highlyefficientprocesseliminatestheneedforpurificationoftheproduct.
  • Easilyscaleupfrompmoltoµmolrange.

Application:
Enzymatic5´adenylationofsingle-strandedDNAlinkersfornextgenerationsequencing(NGS).
5 DNA Adenylation Kit

KitComponents:
10X5DNAAdenylationReactionBuffer
500mMSodiumAcetate(pH6.0@25°C)
100mMMgCl2 
50mMDTT 
1mMEDTA
25mMATP
MthRNALigase

StorageTemperature:-20°C


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