MCLAB/Dh5Alpha/DA100/1 Ea

Description:
DH5a™isthemostfrequentlyusedE.colistrainforroutinecloningapplications.Inadditiontosupportingblue/whitescreeningrecA1andendA1mutationsinDH5a™increaseinsertstabilityandimprovethequalityofplasmidDNApreparedfromminipreps.

Application:

• Highesttransformationefficiency
• Generalcloning
• Blue-whiteselection
• Plasmidisolation

Recommendedstoragecondition:
Thisproductshouldbestoredat-80°C.Thawoniceonlybeforeuse.Donotre-freeze.

Genotype:
F-80dlacZM15(lacZYA-argF)U169recA1endA1hsdR17(rk-,mk+)phoAsupE44-thi-1gyrA96relA1

Reference:
Woodcock,D.M.,Crowther,D.M.,Doherty,J.,Jefferson,S.,DeCruz,E.,Noyer-Weidner,M.,Smith,S.S.,Michael,M.Z.,andGraham,M.W.,Nucl.AcidsRes.(1989)17,3469-3478
CompetentCellType:Chemically

TransformationEfficiency(cfu/μg):
>1x10⁹

Blue-WhietScreening:
Yes

Strain:
K12

ReducesRecombination:
Yes

CloningMethylatedDNA:
No

ImprovesPlasmidQuality:
Yes

PreparingUnmethylatedDNA:
Notsuitable

T1PhageResistant:
No

RecADeficient:
Yes