MCLAB/2X HoTaq™ SYBR Green OneStep RealTime RTPCR Kit/HTSRT100/1 Ea

Description:
RT-PCRiswidelyusedformeasuringgeneexpressionintissuesamplesorcellculturesystems.Traditionally,itisperformedintwoseparatereactionsteps.First-strandcDNAisreverse-transcribedfromtotalRNAorpoly(A)+RNAusingareversetranscriptase.Next,thecDNAisamplifiedbyPCRusingaDNApolymeraseinanotherreaction.

TheMCLAB'sHoTaq™SYBRGreenOne-StepRT-PCRKitallowsresearcherstoperformSYBRGreenbasedRT-qPCRinasinglestepwithinasingletube.ThekitutilizesourownproprietaryengineeredQuantumScriptHDreversetranscriptaseandhot-startTaqDNApolymerase.Noadditionalreagentsorstepsarerequiredonethereactionisinitiated.ThekitenablesthequantificationofspecificRNAtargetswithhighsensitivity,unparalleledconvenience,andwidedynamicrange.

Thetechniquereducestheriskofcross-contaminationandminimizestheuseofreagents.ThismethodisparticularlyusefulforapplicationsinwhichtheexpressionofasmallnumberofgenesthatmustbeanalyzedinmanydifferenttotalRNAsamples,androbustlyamplifyinghigh-abundancetranscriptsfromcrudetotalRNApreparations.

Application:
-GeneExpressionAnalysis
-Genotyping
-Real-TimePCR

Primerandprobedesign: 
Toachievethebestperformance,appropriatesoftware,suchasABIPrimerExpress™,shouldbeused.

RecommendedReactionConditions:
Onecycleat50°Cfor10to30minutes *;
Onecycleat95°Cfor10minutes;
Followedby40cyclesof:95°Cfor15seconds,60°Cfor1minute #;
4°Chold(optional)
* ReactiontimecouldbeadjustedaccordingtoRNAinput.
# Cyclenumberandannealingtemperatureareexperimentdependent.

RecommendedStorageCondition: -20°C