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MCLAB/Bst DNA Polymerase (large fragment)/BPL600/200,000 units188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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MCLAB/Bst DNA Polymerase (large fragment)/BPL600/200,000 units
ForDNAsequencinghighGCregionsandrapidsequencingfromnanogramamountsofDNAtemplates.

Description:
ArecombinantE.colistraincarryingtheBSTDNAPolymerasegene(largefragment).
 

Bst.jpg"
Figure:MCLABBstDNAPolymerase(largefragment)showshigherstrand-displacingpolymeraseactivitythantwoothervendors'products.

Application:
-Sequencingthroughproblematicsecondarystructures

Source:
BstDNAPolymerase(largefragment)isaportionofBacillusstearothermophilusDNAPolymeraseproteinthatcontains5´->3´polymeraseactivity,butlacks5'->3'and3'->5'exonucleaseactivities.

SpecificActivity:120,000U/mg

Suppliedin:
10mMTris-HCl
50mMKCl
1.0mMDTT
0.1mMEDTA
0.1%TritonX-100
50%Glycerol
pH7.5@25°C

Suppliedwith:
10XPCRbufferII

10xPCRBufferll:
200mMTris-HCl
100mMAmmoniumSulfate
100mMKCl
20mMMgSO4
1.0%TritonX-100
pH8.8@25°C

UnitDefinition:
1unitisdefinedastheamountofpolymeraserequiredtoconvert10nmolofdNTPsintoacidinsolublematerialin30minutesat65°C.

RecommendedStorageCondition:-20°C

Reference:
Kiefer,etal.Structure15January1997.5,95-108.


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