MCLAB/Thermostable RNaseH/HTRHOEM/Any Size

Description:
ThermostableRNaseHhasanoptimalactivityabove65°Candcanbeusedupto95°C.TheenzymedegradesRNAinaDNA:RNAhybrid,maximizingsensitivityandselectivitywithoutaffectingDNAorunhybridizedRNA.

Application:
-Highhybridizationstringency
-SpecifichydrolysisofRNAinaDNA:RNAhybrid
-DiagnosticassayofDNAsequencesbyisothermalprobeamplification
-MappingofmRNAstructures

Source:
ArecombinantproteinpurifiedfromE.coli,clonedthegeneencodingtheThermusthermophilusRNaseH.

UnitDefinition:
Oneunitoftheenzymeresultsintheacid-solubilizationof1nmolofpolyadenylicacidinthepresenceofanequimolarconcentrationofpolythymidylicacidin20minutesat45°Cin50mMTris-HCl(pH7.5),100mMNaCl,and10mMMgCl₂.Note:Theunitassayisperformedat45°CbecausethisisoptimalfortheTmofpoly(dT):poly(A).Theoptimaltemperatureformanyapplicationsmaybeconsiderablyhigher.


10XRNaseHReactionBuffer
500mMTris-HCl(pH7.5)
1MNaCl
100mMMgCl₂
pH7.6@25°C

StorageBuffer:
Suppliedin50%Glycerolcontaining50mMTris-HCl(pH7.5),0.1MNaCl,1.0mMDTT,0.1mMEDTA,and0.1%TritonX-100.

RecommendedStorageCondition:-20°C