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MCLAB/Protein G, Alkaline Phosphatase Conjugate/PPG902/1 Ea

RecombinantProteinGisanimmunoglobulin-bindingproteinderivedfromthecellwallofcertainstrainsofb-hemolyticStreptococci.ItbindswithhighaffinitytotheFcportionofvariousclassesandsubclassesofimmunoglobulinsfromavarietyofspecies.ThealbuminandcellsurfacebindingdomainshavebeeneliminatedfromRecombinantProteinGtoreducenonspecificbindingand,therefore,canbeusedtoseparateIgGfromcrudesamples.DuetoitsaffinityfortheFcregionofmanymammalianimmunoglobulins,proteinGisamajorapplicationinpurifyingantibodies.

Description:
RecombinantProteinGisanimmunoglobulin-bindingproteinderivedfromthecellwallofcertainstrainsofb-hemolyticStreptococci.ItbindswithhighaffinitytotheFcportionofvariousclassesandsubclassesofimmunoglobulinsfromavarietyofspecies.ThealbuminandcellsurfacebindingdomainshavebeeneliminatedfromRecombinantProteinGtoreducenonspecificbindingand,therefore,canbeusedtoseparateIgGfromcrudesamples.DuetoitsaffinityfortheFcregionofmanymammalianimmunoglobulins,proteinGisamajorapplicationinpurifyingantibodies.ProteinGisconsideredauniversalreagentinbiochemistryandimmunology.

Applications:

Antibodypurification
Proteinpurification
Isolatesimmunecomplexes
Immunoprecipitation
ImmunoaffinityPurification
WesternBlotting

Source:
PurifiedfromE.colithatcarriestheproteinGgeneofGStreptococci.

Purity:
>98%bySDS-PAGEandHPLCanalyses.ThealbuminbindingdomainaswellascellwallandcellmembranebindingdomainshavebeenremovedtoensurethemaximumspecificIgGbindingcapacity.ThisproteincontainsonlyIgGbindingdomains.

RecommendedStorageConditions:
-20ºC.Avoidfreeze/thawcycles.

Usage:
Underoptimalconditions,1mgproteinGwillbindapproximately5mghumanIgG.OptimalbindingofProteinGtoantibodiesoccursatpH5.0to6.0andcanbeelutedoverapHrangeof2.5to3.0.

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