MCLAB/ChooChoo Cloning Kits/CCK096/1 Ea

Description:
Choo-ChooCloning^TMKitsarehighlyefficientdirectionalPCRcloningkitsforrapid,ligaseandrestrictionenzyme-independentcloningofPCRproducts.TheyallowtocloneanyPCRfragmentintoanylinearizedvectoratanylocation.Bysimplyincubatingonice,theendsofaPCR-generatedDNAfragmentcanbepreciselyfusedintoaDNAvectorwith6bp(ormore)overlappinghomologoussequence.Thesystemisveryrobust.Upto8PCR-generatedDNAfragmentscanbeassembledandclonedintoonepiece(upto10kbinonestep).Thesystemishighlyefficient,with98-100%positivecolonies.

Function:
ThefunctionofChoo-ChooCloning^TMKitsdependonMCLAB'sproprietaryenzymesystems.Thereisnoneedforrestrictionenzymedigestion,ligation,orblunt-endpolishing.Anyextraunwantedbasescanbeeliminated inthefinalconstruct.ThelinearizedvectorcanbegeneratedbyPCRorrestrictionenzymedigestion.ThePCRfragmentscanbegeneratedbyTaqDNApolymeraseorotherhighfidelityDNApolymerases.TheadditionofaAbyTaqDNApolymeraseisnotrequiredandhasnoeffectoncloningefficiency.IfthePCRproductisamplifiedfromaplasmidtemplate,thenitneedstobegel-purifiedtoreducebackground.InadditiontoPCRcloning,Choo-ChooCloning^TMKitsalsohavethefollowingaplications:adaptor,linkerandtagadditionbeforeandaftertheinserts,andgenesynthesis.

Features:
-Cloneanyinsertintoanyvectoryouchooseatanylocation
-Norestrictiondigestion,phosphatasetreatment,orligationrequired
-Multiplefragments(upto8pieces)
-BroadPCRsizes(upto10kb)
-Finalconstructsareseamlesswithnoextraorunwantedbasepairs
-Simple45minutesingle-tubereactiononiceprotocol
-Highefficiencywith>=98%positivecolonies
-Multiplefunctions:compatiblewithadaptor,linkerandtagadditionbeforeoraftertheinserts;compatiblewithgenesynthesis
-Highthroughputapplication

RecommendedStorageConditions:
StoreBox1at-20°C.
StoreBox2at-80°C.

Components:
TheChoo-ChooCloning^TMKitsareavailablein10-,20-,100-,and96-reactionsizes.