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MCLAB/RNase H, E. coli/RNHE100/2,500 units188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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MCLAB/RNase H, E. coli/RNHE100/2,500 units
RNaseHisanendoribonucleasethatdegradestheRNAstrandofRNA/DNAhybridmolecules.

Description:
RNaseHisanendoribonucleasewhichdegradestheRNAstrandofRNA/DNAhybridmolecules.RNaseHdigestionproducesribonucleotidemoleculeswith5'-phosphateand3'-hydroxyltermini.RNAseHisnearlyinactiveagainstsingleordouble-strandedRNAmolecules.

SpecificActivity:625,000U/mg

Application:
-Removalofpoly(A)tailsofmRNAhybridizedtopoly(dT)
-RemovalofmRNAduringsecondstrandcDNAsynthesis

Source:
ArecombinantE.colistraincarryingtheRNAseHgene(rnh)fromE.coli.


Suppliedin:
20mMTris-HCl
100mMKCl
10mMMgCl2
0.1mMEDTA
0.1mMDTT
50%Glycerol
pH7.9@25°C

Suppliedwith:
10xRNAseHBuffer

10xRNAseHBuffer:
500mMTris-HCl
750mMKCl
30mMMgCl2
100mMDTT
pH8.3@25°C

UnitDefinition:
1unitisdefinedastheamountofenzymethatwillhydrolyze1nmolofRNAfroman3H-labeledDNA:RNAhybridmoleculeintoacid-solublematerialin20minutesat37°C.

RecommendedStorageCondition:-20°C


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