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MCLAB/MCMag PCR Purification Kit/MCPP60/1 Ea
TheMCMagPCRpurificationsystemutilizessolid-phasereversibleimmobilization(SPRI)paramagneticbeadtechnologyforPCRampliconpurification.

Description
TheMCMagPCRpurificationsystemutilizessolid-phasereversibleimmobilization(SPRI)paramagneticbeadtechnologyforPCRampliconpurification.TheMCMagbeadsarepre-formulatedwithanoptimizedbuffertoselectivelybindDNAfragmentsof100bpandlarger.Excesssalts,enzymes,primersandnucleotidescanberemovedthroughasimplewashingprocedure.TheMCMagPCRPurificationsystemisfullyadaptabletoautomation.

Applications

  • PCR
  • Sequencing
  • FragmentAnalysis
  • Genotyping
  • Cloning
  • PrimerWalking

MaterialSupplied

  • MCMagPCRPurificationbeads
  • Storeat4°Cuponarrival,forupto18months
  • Shakethereagentwelltoahomogenousappearancebeforeuse

Specifications

Table1.Availableproductsizes

MCMagPCRPurification

CatalogNumber

5mL

MCPP-5

60mL

MCPP-60

450mL

MCPP-450


Table2.NumberofPCRreactionspurifiedwith96-and384-wellformats

ProductSize

PCRreactionvolume(µL) 96-wellformat

5mL

60mL

450mL

10

278rxns

3332rxns

25000rxns

20

139rxns

1666rxns

12500rxns

50

56rxns

667rxns

5000rxns

100

28rxns

334rxns

2500rxns

PCRreactionvolume(µL)  384-wellformat

5

556rxns

6667rxns

50000rxns

7

397rxns

4762rxns

35714rxns

10

278rxns

3333rxns

20000rxns

14

198rxns

2381rxns

17857rxns


MaterialsNotSupplied

  • ReactionPlate
  • McBeadMagneticPlate
  • PlateSeals,AdhesiveorHeat
  • WashSolution(70%ethanol)
  • ElutionBuffer
    • Water
    • Tris-Acetate(10mMpH8.0)
    • TEBuffer(10mMTris-AcetatepH8.0,1mMEDTA

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