37%ofallcellculturesupernatants,andalmostallofconfluentcellculturesupernatants,inhibitPCR.ProficiencystudieshavedemonstratedtheimportanceoffurtherDNAextractionforasensitivePCRresultsevenforunremarkablesamplematerials.
TheVenor®GeMSamplePreparationKitprovidessampleshighlycompatIBLewithMinervaBiolabsVenor®GeMMycoplasmaPCRkits.AspecialprotocolallowsthepurificationofthesamplesinordertomeetthehighdemandsoftheEP2.6.7.ThesampleDNAiselutedinavolumeof50µlinahighconcentrationandpurityformaximumsensitivityofthePCR.ThekitworkswithDNAextractioncolumnswhicharedirectlysuitableforthetreatmentofupto250µlofyoursample.
ForDNAextractionfrom:
Cellculturesupernatantsincludingcellsandcelldebris
ATMPs
Sampleswithpolymersandglycerine(e.g.cryoconservationmedia)
Cellculturemedia
Proteinsolutions,incl.antibodiesandenzymes.
Spincolumns
Collectiontubes
Conditioner,Washandelutionbuffers
Ethanolisrequiredforthereconstitutionofthewashbufferbutnotincludedinthekit.Microcentrifugevials(1.5ml)areneededtoDNAelution.
Atproteinconcentrationsabove10mg/mlinthesamplepretreatmentwithProteinaseKisstronglyrecommended(Cat.-No.11-9905).
Storeandshipmentatambienttemperature.
SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn