Venor®GeMClassicallowsfast,reliableandtime-savingroutinemonitoringofmycoplasmacontaminationbyPCR.
Forconventional,endpointPCR
ApplicableinresearchandindustryfordirecttestingofcellculturesandBIOLOGicals.Intendedforresearchuseonly.Notrecommendedforclinicaldiagnostics.
Lyophilizedprimersetsandnucleotides
10xreactionbufferoptimizedforMBTaqDNAPolymerase
LyophilizedpositivecontrolDNA
Lyophilizedinternalamplificationcontrol
Primersetsandnucleotidesarepreparedinaliquotsof25tests.
GelelectrophoresisatendpointofPCR
Polymerase.Wehighlyrecommendourreliablehot-startMBTaqDNAPolymerase,Cat.-No.53-0050/100/200/250. PCRreactiontubes
OptionalforprocessvalidationandEP2.6.7complianttesting: InternalControlDNAextra(4vialsfor300µleachofinternalamplificationcontrol;Cat.No.11-1905) 10CFU™SensitivityStandardsavailableforallEPlistedmycoplasmaspecies
RegularPCRcycler
AgarosegelelectrophoresisandDNAstainingsystem
Pipettingequipment
Tubecentrifuge
Componentsaremaintainableat+2to+8°Cforatleast6months.Afterrehydratisationthereagentsmustbestoredat-18°C.
UseofVenor®GeMClassicforQAtestingofbiologicalslikemasterandworkingcellbanks,autologouscells,culturemedia,bulkharvestandfinalproducttestingaccordingtoEP2.6.7isapplicableafterappropriatesamplepreparationandprocessvalidation.
Fig.AmplifiedPCRproductsarevisualizedbystandardgelelectrophoresis.
SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn