Minervabiolabs/Meat ID™ Screen/1201005/5 tests

Meat ID™ Screen is a kit for the fast and reliable identification of several animal species (Goat, Sheep, Pork, Horse, Beef, Donkey, Fish, Chicken, Turkey and Camel) in meat by qPCR in a single parallel run.

The identification of different meats, especially in minced meat products is a serious task in food safety and from and ethical perspective, especially for Muslims. Authentication of forbidden or none declared ingredients such as pork or substandard meat is essential to ensure confidence in the supply chain and regulatory compliance.

Meat ID™ Screen is available for rapid and reliable analysis from various matrices including raw, or even highly processed and cooked meat products where the DNA may be significantly degraded. It is possible to identify relevant species down to a threshold level of 0.5 % with a semi-quantitative result.

Type of PCR

TaqMan® Assay based on FAM™ and HEX™ labeled probes.

Recommended Use / Scope

Can be used in research and industry for detection of animal species (Goat, Sheep, Pork, Horse, Beef, Donkey, Fish, Chicken, Turkey, Camel, Zebra) in meat and other foods by qPCR. For research use only! Do not use for clinical diagnostic or testing of patient samples.

Target sequence is a mitochondrial gene (cytochrome b), which is available in multiple copies per cell, enabling successful detection of minute amounts of target DNA!

Package Sizes

• Cat. No. 12-01-005 5 tests
  Cat. No. 12-01-020 20 tests
  Cat. No. 12-01-040 40 tests

Result Evaluation

cycler based, real-time PCR

Sensitivity

Down to a threshold level of 0.5% with a semi-quantitative result.

Content

Primer sets, nucleotides, polymerase and internal control DNA, lyophilized, filled in 0.2 ml PCR reaction tubes (Stripes). Rehydration buffer, Positive control, PCR grade water.

Sample Requirements

DNA can be extracted from sample material using DNA extraction kits e.g. ExtractNow™ DNA Mini Kit (Art.-Nr. 608-1050) or user method.

Required Consumables

Pipette tips, 1.5 ml reaction tubes

Required Lab Devices

qPCR cycler with FAM™ and HEX™ filter, benchtop centrifuge, racks for tubes, variable microliter pipettes, sterile filter tips

Time to result

approx. 90 minutes

Shelf Life and Storage

Components can be stored at +2 to +8 °C for at least 12 months. After rehydration reagents must be stored at -18 °C.

Fig. 1: Sensitivity of the Meat ID™ Screen, parameter Horse.

Fig. 2: Detection of 1% w/w horse meat in respective meat background