Minervabiolabs/Venor®GeM Advance/117240/240 tests

Venor®GeM Advance contains PCR reaction tubes pre-coated with all PCR reagents to reduce the total assay time without need to prepare aliquots of a master mix. For additional convenience the gel loading buffer and dye are already included in the reaction buffer. After thermal cycling the PCR can be loaded directly on the agarose gel.

Type of PCR

For conventional, endpoint PCR

Scope of Application

Applicable in research for direct testing of cell cultures and cell culture-derived biologicals. Intended for research use only. Not recommended for clinical diagnostics or testing of human samples.

Kit Components

Primer sets, nucleotides, polymerase and internal control DNA, lyophilized, filled in 0.2 ml PCR reaction tubes
Rehydration buffer including gel loading buffer and running dye
Positive controls, lyophilized, filled in 0.2 ml PCR reaction tubes
PCR grade water

Package Sizes

• Cat. No. 11-7024 24 Tests
  Cat. No. 11-7048 48 Tests
  Cat. No. 11-7096 96 Tests
  Cat. No. 11-7240 240 Tests

Result evaluation

Gel electrophoresis at endpoint of PCR
Required Consumables

Agarose and DNA-specific dye for gel staining

Required lab devices

Regular block-heated PCR cycler
Agarose gel electrophoresis and gel documentation system
Pipetting equipment
Tube centrifuge

Shelf Life and Storage

Kit components are maintainable at +2 to +8 °C for at least 6 months. After rehydratisation of the individual PCR tubes the reagents must be used within 1 hour.

EP 2.6.7 compliance

No

Fig. Amplified PCR products are visualized by standard gel electrophoresis.