Minervabiolabs/Food Control™ LFA/110310025/

FoodControl™LFAisaPCR-kitforfastandreliabledetectionoffoodbornepathogensviaconventionalPCRincombinationwithourreliableLateralFlowAssay(LFA)evaluationtechnology.

DetectingbacterialcontaminationisakeyaspectinfoodmicroBIOLOGy.Consequently,microbiologicalqualitycontrolprogramsareintensifiedthroughoutfoodchainproductioninordertominimizetheriskofintoxicatingconsumers.ReliABIlity,speedandrobustnessofatestsystemtodetectthepresenceorabsence,oreventhedegreeofcontaminationwithpathogens,becomesincreasinglyimportant.FoodControl™LFAisadiagnosticsystemforeasynon-quantitativedetectionofpresenceorabsenceoffoodbornepathogensinagriculturalorfoodindustryviaconventionalPCRandLateralFlowAssay(LFA)evaluationtechnology.

TypeofAssay

TheassayisbasedonaonetubeamplificationwithaconventionalPCRcyclerandasubsequentprobespecifichybridizationtechnique.Theresultinginterpretationisasimplereadoutofcolouredbarsonalateralflowstripe.

RecommendedUse/Scope

Canbeusedinresearchandindustryfordetectionofpathogeniccontaminationinfoodoragriculturalproducts.Forresearchuseonly!Donotuseforclinicaldiagnosticortestingofpatientsamples.

ThekitisavailableforthedetectionofthefollowingmicroorganismsviaconventionalPCR:

Features

PackageSizes

Packagesize25reactionseach:

• Cat.No.11-03-01-025Salmonellaenterica 
• Cat.No.11-03-02-025Yersiniaenterocolitica
• Cat.No.11-03-03-025Shigellaspp.
• Cat.No.11-03-04-025Campylobacterspp. 
• Cat.No.11-03-05-025Clostridiumperfringens
• Cat.No.11-03-06-025ShigaToxin1
• Cat.No.11-03-07-025ShigaToxin2
• Cat.No.11-03-08-025EscherichiacoliO157
• Cat.No.11-03-09-025EscherichiacoliO104
• Cat.No.11-03-10-025Listeriaspp.
• Cat.No.11-03-11-025Listeriamonocytogenes
• Cat.No.11-03-12-025Salmonellaspp.

ResultEvaluation

LateralFlowStripe.

Sensitivity

Downto10DNAcopies/assay.

Content

PCRMix,rehydrationbuffer,PCRgradewater,positivecontrol,speciesspecificprimer,speciesspecificprobe,LFAstripe,
LFArunningbuffer

SampleRequirements

TheFoodControl™kitsaredesignedforthePCRbaseddetectionofbacterialDNA.IsolatedtotalDNAfrompotentiallycontaminatedfoodserveshereasstartingmaterial,typicallyafterpre-cultivationofthesamplegrowthmedium.

Timetoresult

150minutes

RequiredConsumables

PCRreactiontubes,2.0mlreactiontubes

Optional:DNAcanbeextractedfromenrichmentbrothusingtheDNAextractionkitExtractNow™FoodControl(Cat.No.609-1050)orusermethod.

RequiredLabDevices

Racksforreactiontubes,benchtopcentrifugeforreactiontubesorPCRplates,vortex,variablemicroliterPipettes(1µl–1000µl),sterilefiltertips,anyconventionalPCRcycler.

ShelfLifeandStorage

Componentscanbestoredat+2to+8°Cforatleast12months.Afterrehydrationthereagentsmustbestoredat-18°C.

Compliance

No,certificationinprogress.