Minervabiolabs/Venor®GeM Sample Preparation Kit/561050/50 extractions

37%ofallcellculturesupernatants,andalmostallofconfluentcellculturesupernatants,inhibitPCR.ProficiencystudieshavedemonstratedtheimportanceoffurtherDNAextractionforasensitivePCRresultsevenforunremarkablesamplematerials.

TheVenor®GeMSamplePreparationKitprovidessampleshighlycompatIBLewithMinervaBiolabsVenor®GeMMycoplasmaPCRkits.AspecialprotocolallowsthepurificationofthesamplesinordertomeetthehighdemandsoftheEP2.6.7.ThesampleDNAiselutedinavolumeof50µlinahighconcentrationandpurityformaximumsensitivityofthePCR.ThekitworkswithDNAextractioncolumnswhicharedirectlysuitableforthetreatmentofupto250µlofyoursample.

ScopeofApplication

ForDNAextractionfrom:

Cellculturesupernatantsincludingcellsandcelldebris
ATMPs
Sampleswithpolymersandglycerine(e.g.cryoconservationmedia)
Cellculturemedia
Proteinsolutions,incl.antibodiesandenzymes.

KitComponents

Spincolumns
Collectiontubes
Conditioner,Washandelutionbuffers

RequiredConsumables

Ethanolisrequiredforthereconstitutionofthewashbufferbutnotincludedinthekit.Microcentrifugevials(1.5ml)areneededtoDNAelution.

Atproteinconcentrationsabove10mg/mlinthesamplepretreatmentwithProteinaseKisstronglyrecommended(Cat.-No.11-9905).

PackageSizes

• Cat.No.56-101010extractions
• Cat.No.56-105050extractions
• Cat.No.56-1200200extractions

Storage

Storeandshipmentatambienttemperature.