Minervabiolabs/Meat ID™ Screen/1201040/40 tests

MeatID™Screenisakitforthefastandreliableidentificationofseveralanimalspecies(Goat,Sheep,Pork,Horse,Beef,Donkey,Fish,Chicken,TurkeyandCamel)inmeatbyqPCRinasingleparallelrun.

Theidentificationofdifferentmeats,especiallyinmincedmeatproductsisaserioustaskinfoodsafetyandfromandethicalperspective,especiallyforMuslims.Authenticationofforbiddenornonedeclaredingredientssuchasporkorsubstandardmeatisessentialtoensureconfidenceinthesupplychainandregulatorycompliance.

MeatID™Screenisavailableforrapidandreliableanalysisfromvariousmatricesincludingraw,orevenhighlyprocessedandcookedmeatproductswheretheDNAmaybesignificantlydegraded.Itispossibletoidentifyrelevantspeciesdowntoathresholdlevelof0.5%withasemi-quantitativeresult.

TypeofPCR

TaqMan®AssaybasedonFAM™andHEX™labeledprobes.

RecommendedUse/Scope

Canbeusedinresearchandindustryfordetectionofanimalspecies(Goat,Sheep,Pork,Horse,Beef,Donkey,Fish,Chicken,Turkey,Camel,Zebra)inmeatandotherfoodsbyqPCR.Forresearchuseonly!Donotuseforclinicaldiagnosticortestingofpatientsamples.

Targetsequenceisamitochondrialgene(cytochromeb),whichisavailableinmultiplecopiespercell,enablingsuccessfuldetectionofminuteamountsoftargetDNA!

PackageSizes

• Cat.No.12-01-0055tests
  Cat.No.12-01-02020tests
  Cat.No.12-01-04040tests

ResultEvaluation

cyclerbased,real-timePCR

Sensitivity

Downtoathresholdlevelof0.5%withasemi-quantitativeresult.

Content

Primersets,nucleotides,polymeraseandinternalcontrolDNA,lyophilized,filledin0.2mlPCRreactiontubes(Stripes).Rehydrationbuffer,Positivecontrol,PCRgradewater.

SampleRequirements

DNAcanbeextractedfromsamplematerialusingDNAextractionkitse.g.ExtractNow™DNAMiniKit(Art.-Nr.608-1050)orusermethod.

RequiredConsumables

Pipettetips,1.5mlreactiontubes

RequiredLabDevices

qPCRcyclerwithFAM™andHEX™filter,benchtopcentrifuge,racksfortubes,variablemicroliterpipettes,sterilefiltertips

Timetoresult

approx.90minutes

ShelfLifeandStorage

Componentscanbestoredat+2to+8°Cforatleast12months.Afterrehydrationreagentsmustbestoredat-18°C.

Fig.1:SensitivityoftheMeatID™Screen,parameterHorse.

Fig.2:Detectionof1%w/whorsemeatinrespectivemeatbackground