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Innovative Research/Human Endostatin ELISA Kit/IRKTAH5096/1 Ea188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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Innovative Research/Human Endostatin ELISA Kit/IRKTAH5096/1 Ea

ProductDescription

HumanEndostatinELISAKit-1Kit(96Wells)
OurHumanEndostatinELISAKitisasandwichELISAintendedforthequantitativedeterminationofhumanEndostatinincellculturesupernates,serum,plasma(heparin,EDTA)andhumanmilk.

Endostatinisanaturally-occurring20-kDaC-terminalfragmentderivedfromtypeXVIIIcollagen.Itisreportedtoserveasananti-angiogenicagent,similartoangiostatinandthrombospondin.AnditisproducedbyproteolyticcleavageofcollagenXVIII,amemberofthemultiplexinfamilythatischaracterizedbyinterruptionsinthetriplehelixcreatingmultipledomains,byproteasessuchascathepsins.Usingagenomiccloneasaprobeforfluorescenceinsituhybridization,EndostatinwasmappedtheCOL18A1geneto21q22.3.Byimmunoprecipitationanalysisusingmembranefractionsofhumanmammaryepithelialcells,Itshowedthatendostatinspecificallyboundtocellsurfacenucleolinwithhighaffinity.BlockageofnucleolinwithneutralizingantibodyorknockdownofnucleolinbyRNAinterferencecounteredtheantiendothelialactivityofendostatinandabrogateditsantiangiogenicandantitumoractivityinvivo.

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