AdditionalDonorInformation:
AntigenSpecificInterferon-γSecretion
AntigenIFN-γConcentration(pg/mL)
Tcellsonlycontrols6.4
plusAPC13.9
plusAPC+HLA-B35EBNA15.7
75,000Tcellswereplatedina96-wellround-bottomplatealoneorinthepresenceof10,000APCs(B-LCLsexpressingHLA-B35andHLA-A2)aloneorinthepresenceof1μg/mLHLA-B35restrictedEBNApeptide(HPVGEADYFEY),HLA-B35restrictedCMVpp65peptide(IPSINVHHY)orHLA-A2restrictedCMVpp65peptide(NLVPMVATV).Afteranovernight(18-24h)incubationperiod,supernatantwascollectedfromeachwell.IFN-γconcentrationwasanalyzedusingtheMesoScaleDiscoveryIFN-γassay.
Healthyyoungdonortakingnomedications.HeisCMVSEROpositiveandFcgammaRIIIaF/F.
SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn