Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human MED30/ TRAP25.参阅Abcam的专有抗源政策 (Peptide available as ab71170.)
This antibody gave a positive signal in the following Human Lysates:HCT 116 Whole CellHEK293 Whole CellTE 671 Whole CellThis antibody gave a positive result when used in the following methanol fixed cell lines: HepG2
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\"应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
ICC/IF image of ab49456 stained HepG2 cells. The cells were 100% methanol fixed (5 min) then permeabilised using 0.1% PBS-Triton and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to further permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab49456 at 1 g/ml overnight at +4 C. The secondary antibody (pseudo-colored green) was Alexa Fluor 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43 M for 1hour at room temperature.
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab49456 overnight at 4 C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
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