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MED30 / TRAP25抗体(ab49456)| Abcam中文官网

Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human MED30/ TRAP25.参阅Abcam的专有抗源政策 (Peptide available as ab71170.)
This antibody gave a positive signal in the following Human Lysates:HCT 116 Whole CellHEK293 Whole CellTE 671 Whole CellThis antibody gave a positive result when used in the following methanol fixed cell lines: HepG2
常规说明

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q As


存放说明 Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液 pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS

Batches of this product that have a concentration
The Abpromise guarantee

Abpromise™承诺保证使用ab49456于以下的经测试应用

\"应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。


Thyroid hormone receptor-associated protein 6 antibody Thyroid hormone receptor-associated protein complex 25 kDa component antibody TRAP/Mediator complex component TRAP25 antibody Trap25 antibody see all

ICC/IF image of ab49456 stained HepG2 cells. The cells were 100% methanol fixed (5 min) then permeabilised using 0.1% PBS-Triton and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to further permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab49456 at 1 g/ml overnight at +4 C. The secondary antibody (pseudo-colored green) was Alexa Fluor 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43 M for 1hour at room temperature.


All lanes : Anti-MED30 / TRAP25 antibody (ab49456) at 1 µg/ml

Lane 1 : HCT 116 (Human Colorectal Carcinoma) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 3 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 20 kDa
Observed band size: 22 kDa why is the actual band size different from the predicted?
Additional bands at: 19 kDa, 85 kDa. We are unsure as to the identity of these extra bands.


Exposure time: 8 minutes


This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab49456 overnight at 4 C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.


All lanes : Anti-MED30 / TRAP25 antibody (ab49456) at 1 µg/ml

Lane 1 : HCT 116 (Human Colorectal Carcinoma) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 3 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 20 kDa
Observed band size: 19 kDa why is the actual band size different from the predicted?
Additional bands at: 250 kDa, 75 kDa. We are unsure as to the identity of these extra bands.


Exposure time: 18 minutes

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ab49456 尚未被引用在任何文献中。


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