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Prosci/Activin A Recombinant Protein/96-784/25 ug

Specifications

SPECIES:Human
SOURCESPECIES:HEK293cells
SEQUENCE:Gly311-Ser426
FUSIONTAG:TagFree
TESTEDAPPLICATIONS:ELISA,WB
APPLICATIONS:ThisrecombinantproteincanbeusedforE,WB.Forresearchuseonly.
BIOLOGICALACTIVITY:MeasuredbyitsbindingABIlityinafunctionalELISA.ImmobilizedHumanActivinA,TagFreeat10ug/mL(100uL/well)canbindHumanACVR1B,FcTagwithalinearrangeof0.3-2.5ug/mL(QCtested).

Properties

PURITY:>95%asdeterminedbySDS-PAGE.

Endotoxinlevelislessthan1.0EUperugbytheLALmethod.
PREDICTEDMOLECULARWEIGHT:13kDa
PHYSICALSTATE:Lyophilized
BUFFER:0.056%TFAin60%ACN
STORAGECONDITIONS:LyophilizedProteinshouldbestoredat-20˚Corlowerforlongtermstorage.Uponreconstitution,workingaliquotsshouldbestoredat-20˚Cor-70˚C.Avoidrepeatedfreeze-thawcycles.

AdditionalInfo

ALTERNATENAMES:ActivinA,INHBA
ACCESSIONNO.:AAH07858.1

Background

Activinandinhibinaretwocloselyrelatedproteincomplexesthathavealmostdirectlyoppositebiologicaleffects.ActivinenhancesFSHbiosynthesisandsecretion,andparticipatesintheregulationofthemenstrualcycle.Manyotherfunctionshavebeenfoundtobeexertedbyactivin,includingrolesincellproliferation,differentiation,apoptosis,metabolism,homeostasis,immuneresponse,woundrepair,andendocrinefunction.ConverselyinhibindownregulatesFSHsynthesisandinhibitsFSHsecretion.Activinsarenonglycosylatedhomodimersorheterodimersofvariousbetasubunits(betaA,betaB,betaC,andbetaEinmammals),whileInhibinsareheterodimersofauniquealphasubunitandoneofthebetasubunits.ActivinAisawidelyexpressedhomodimeroftwobetaAchains.ThebetaAsubunitcanalsoheterodimerizewithabetaBorbetaCsubunittoformActivinABandActivinAC,respectively.The14kDamaturehumanbetaAchainshares100%aminoacidsequenceidentitywithbovine,feline,mouse,porcine,andratbetaA.
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