| SPECIES: | Human |
|---|---|
| SOURCESPECIES: | HEK293cells |
| SEQUENCE: | Gly311-Ser426 |
| FUSIONTAG: | TagFree |
| TESTEDAPPLICATIONS: | ELISA,WB |
| APPLICATIONS: | ThisrecombinantproteincanbeusedforE,WB.Forresearchuseonly. |
| BIOLOGICALACTIVITY: | MeasuredbyitsbindingABIlityinafunctionalELISA.ImmobilizedHumanActivinA,TagFreeat10ug/mL(100uL/well)canbindHumanACVR1B,FcTagwithalinearrangeof0.3-2.5ug/mL(QCtested). |
| PURITY: | >95%asdeterminedbySDS-PAGE. Endotoxinlevelislessthan1.0EUperugbytheLALmethod. |
|---|---|
| PREDICTEDMOLECULARWEIGHT: | 13kDa |
| PHYSICALSTATE: | Lyophilized |
| BUFFER: | 0.056%TFAin60%ACN |
| STORAGECONDITIONS: | LyophilizedProteinshouldbestoredat-20˚Corlowerforlongtermstorage.Uponreconstitution,workingaliquotsshouldbestoredat-20˚Cor-70˚C.Avoidrepeatedfreeze-thawcycles. |
| ALTERNATENAMES: | ActivinA,INHBA |
|---|---|
| ACCESSIONNO.: | AAH07858.1 |
| Activinandinhibinaretwocloselyrelatedproteincomplexesthathavealmostdirectlyoppositebiologicaleffects.ActivinenhancesFSHbiosynthesisandsecretion,andparticipatesintheregulationofthemenstrualcycle.Manyotherfunctionshavebeenfoundtobeexertedbyactivin,includingrolesincellproliferation,differentiation,apoptosis,metabolism,homeostasis,immuneresponse,woundrepair,andendocrinefunction.ConverselyinhibindownregulatesFSHsynthesisandinhibitsFSHsecretion.Activinsarenonglycosylatedhomodimersorheterodimersofvariousbetasubunits(betaA,betaB,betaC,andbetaEinmammals),whileInhibinsareheterodimersofauniquealphasubunitandoneofthebetasubunits.ActivinAisawidelyexpressedhomodimeroftwobetaAchains.ThebetaAsubunitcanalsoheterodimerizewithabetaBorbetaCsubunittoformActivinABandActivinAC,respectively.The14kDamaturehumanbetaAchainshares100%aminoacidsequenceidentitywithbovine,feline,mouse,porcine,andratbetaA. |
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