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| SPECIESREACTIVITY: | Human,Mouse,Rat |
|---|---|
| HOMOLOGY: | Predictedspeciesreactivitybasedonimmunogensequence:Pig:(100%),Bovine:(100%) |
| TESTEDAPPLICATIONS: | ELISA,ICC,IF,WB |
| APPLICATIONS: | Aak1antibodycanbeusedfordetectionofAak1byWesternblotat1-2μg/mL.Antibodycanalsobeusedforimmunocytochemistrystartingat5μg/mL.Forimmunofluorescencestartat20μg/mL. |
| USERNOTE: | Optimaldilutionsforeachapplicationtobedeterminedbytheresearcher. |
| POSITIVECONTROL: | 1)Cat.No.1466-RatSpleenTissueLysate |
| 2)Cat.No.17-208-A-20CellSlide | |
| 3)Cat.No.10-301-HumanBrainTissueSlide | |
| IMMUNOGEN: | Aak1antibodywasraisedagainsta20aminoacidsyntheticpeptideneartheaminoterminusofthehumanAak1. Theimmunogenislocatedwithinaminoacids190-240ofAak1. |
| HOSTSPECIES: | Rabbit |
| PURIFICATION: | Aak1Antibodyisaffinitychromatographypurifiedviapeptidecolumn. |
|---|---|
| PHYSICALSTATE: | Liquid |
| BUFFER: | Aak1AntibodyissuppliedinPBScontaining0.02%sodiumazide. |
| CONCENTRATION: | 1mg/mL |
| STORAGECONDITIONS: | Aak1antibodycanbestoredat4˚Cforthreemonthsand-20˚C,stableforuptooneyear.Aswithallantibodiescareshouldbetakentoavoidrepeatedfreezethawcycles.Antibodiesshouldnotbeexposedtoprolongedhightemperatures. |
| CLONALITY: | Polyclonal |
| ISOTYPE: | IgG |
| CONJUGATE: | Unconjugated |
| ALTERNATENAMES: | Aak1Antibody:KIAA1048,AP2-associatedproteinkinase1,Adaptor-associatedkinase1 |
|---|---|
| ACCESSIONNO.: | NP_055726 |
| PROTEINGINO.: | 148277037 |
| OFFICIALSYMBOL: | AAK1 |
| GENEID: | 22848 |
| BACKGROUND: | Aak1Antibody:AP2-associatedproteinkinase1(Aak1)isamemberoftheArk1/Prk1subfamilyofSer/Thrproteinkinasesthatarethoughttoregulateendocytosisbyphosphorylatingtheaccessoryendocyticcomponents.Aak1interactswithandphosphorylatesthemu2subunitoftheAP-2complex,whichpromotesbindingoftheAP-2totyrosinebased(Yxxphi)internalizationmotif-containingreceptorsandsubsequentreceptorendocytosis.AtleasttwoisoformsofAak1areknowntoexist;thelongerisoformcontainsanextendedcarboxy-terminusthatcontainsanadditionalclathrin-bindingdomain.OverexpressionofthislongisoformorAak1depletionbyRNAinterferenceimpairstransferrinrecyclingfromtheearly/sortingendosome,suggestingthatAak1functionsatmultiplestepsoftheendosomalpathwaybyregulatingtransferrininternalizationanditsrecyclingbacktotheplasmamembrane. |
|---|---|
| REFERENCES: | 1)ConnorSDandSchmidSL.Identificationofanadaptor-associatedkinase,AAK1,asaregulatorofclathrin-mediatedendocytosis.J.CellBiol.2002;156:921-9. | 2)SmytheEandAyscoughKR.TheArk1/Prk1familyofproteinkinases.Regulatorsofendocytosisandtheactinskeleton.EMBORep.2003;4:246-51. | 3)RicottaD,ConnorSD,SchmidSL,etal.PhosphorylationoftheAP2m2subunitbyAAK1mediateshighaffinitybindingtomembraneproteinsortingsignals.J.CellBiol.2002;156:791-5. | 4)ConnorSDandHendersonDM.AnovelAAK1splicevariantfunctionsatmultiplestepsoftheendocyticpathway.Mol.Biol.Cell2007;18:2698-706. |
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