SYBR™ Green I Nucleic Acid Gel Stain, 10,000X concentrate in DMSO

SYBRGreenINucleicAcidGelStainisoneofthemostsensitivestainsavailablefordetectingdouble-strandedDNA(dsDNA)inagaroseandpolyacrylamidegels.BecauseSYBRGreenIhasgreatersensitivityfordsDNA,itisespeciallyusefulforassayswherethepresenceofcontaminatingRNAorssDNAmightobscureresults.Withexceptionallylowbackgroundfluorescenceandspectralcharacteristicsthatcloselymatchlightsourcesandfiltersetsinexistinginstruments,SYBRGreenIstainisidealforusewithlaserscanners.SYBRGreenIstainprovides:

SensitivityatleastfourtimesgreaterthanethidiumbromideforDNAinagarosegels
Easeofusegelssoakedindilutedstaincanbevisualizedwithoutdesalting
CompatibilitywithUVtransIlluminators,geldocumentationsystems,andlaserscanners
Flexibilityforusewithabroadrangeofapplications,includingDNAtyping,PCR-basedassays,DNAdamageassays,analysisofcomplexsamples,andreal-timePCRdetection

Productuse
OnemLstains100minigels.Variouspacksizesareavailable.
HL-60cellsinacometassay.
CometassaywithSYBR®GreenINucleicAcidGelStain(Cat.No.S7563,S7567,S7585).DNAfragmentationassociatedwithoxidativeDNAdamagewasvisualizedusingTrevigensCometAssaykit.HL-60cellsweretreatedwithhydrogenperoxideandimmobilizedontoaTrevigenCometSlideforanalysis.Thecellsweregentlylysed,washed,andtreatedwithendonuclease.SlidesweresubjectedtoelectrophoresisinalkalineelectrophoresisbufferandstainedwithSYBR®GreenIstain."data-omni-action="Viewfigure">

HL-60cellsinacometassay.

DNAmolecularweightladdersstainedwithSYBR®GreenINucleicAcidGelStain.
DNAmolecularweightladdersthatwereelectrophoresedona1%agarosegelwerestainedfor30minwitha1:10,000dilutionofSYBR®GreenINucleicAcidGelStain(Cat.No.S7563,S7567,S7585).Lanes1and8containHindIII–cutDNA;lanes2and7,HaeII–cutfX174RFDNA;lanes3and6,1kilobasepairDNAladder(LifeTechnologies);lane4,100BasePairDNALadder(LifeTechnologies);lane5,EcoRI–cutpUC19DNAmixedwithPstI–cutfX174RFDNA.Gelstainingwasvisualizedusing254nmepi-illumination."data-omni-action="Viewfigure">

DNAmolecularweightladdersstainedwithSYBR®GreenINucleicAcidGelStain.

Comparisonofsingle-strandedoligonucleotidedetectionusingSYBR®GreenINucleicAcidGelStainandethidiumbromide.
Identicalthree-folddilutionsofasynthetic,single-stranded24-merwereelectrophoresedon10%polyacrylamidegels.Gelswerestainedfor30minwitha1:10,000dilutionofSYBR®GreenINucleicAcidGelStain(Cat.No.S7563,S7567,S7585)andnotdestained(panelsAandB)orwith5µg/mLethidiumbromide(Cat.No.E1305,E3565)for30minanddestainedforafurther30mininwater(panelC).Gelstainingwasvisualizedusing254nmepi-illumination(panelA)or300nmtransillumination(panelsBandC)andthenphotographedusingPolaroid667black-and-whiteprintfilmandaSYBR®photographicfilter(Cat.No.S7569,panelsAandB)oranethidiumbromidegelstainphotographicfilter(panelC)."data-omni-action="Viewfigure">

Comparisonofsingle-strandedoligonucleotidedetectionusingSYBR®GreenINucleicAcidGelStainandethidiumbromide.

ElectrophoreticbandshiftassayusingSYBR®GreenINucleicAcidGelStain.
Theassociationbetweena208-bpfragmentpurifiedfromAvaI–digestedplasmidp5S208-12andamutantrestrictionendonuclease(EcoRI/Gln111)wasanalyzedona4%nativepolyacrylamidegel.Samplescontainingapproximately50ngtotalfragmentsandvariousamountsofthemutantenzymeweresubjectedtoelectrophoresisandstainedwithSYBR®GreenINucleicAcidGelStain.Gelstainingwasvisualizedusing254nmepi-illuminationandthenphotographedusingPolaroid667black-and-whiteprintfilmandaSYBR®photographicfilter(Cat.No.S7569).Lane1containsHaeIII-digestedFX174RFDNAmarkers;lanes2through9contain0,0.05,0.1,0.2,0.4,0.6,0.8and0µMEcoRI/Gln111;lane10containsHhaI-digestedplasmidp5S208-12asasizestandard."data-omni-action="Viewfigure">

ElectrophoreticbandshiftassayusingSYBR®GreenINucleicAcidGelStain.

Suppliedasa10,000XconcentrateinDMSO.Storeat-20C,protectedfromlightinadessicator

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