Trevigen/Cultrex® Poly-L-Lysine/3438-100-01/100 ml, 2 x 100 ml

Description

Poly-L-Lysine,ahighlypositivelychargedsyntheticaminoacidchain,iscommonlyusedasacoatingagenttopromotecelladhesionincultureontissueculture-treatedplasticorglasssurfaces.Moreover,poly-L-Lysinecontainspolymersinthe70,000-150,000Darangeandpromotestheproliferationanddifferentiationofavarietyofneuronalcelllines.Thissolutionisprovidedsterilefilteredandreadytouseat0.01%.

Qualitycontrolspecifications:

• Nobacterialorfungalgrowthdetectedafterincubationat37°Cfor14daysfollowingUSPsterilitytestingguidelines.
• Endotoxinconcentration≤20EU/mLbyLALassay.
• Proteinconcentrationof0.01%.
• Providedinphosphate-bufferedsaline(PBS).
• BIOLOGicalactivitydeterminedforeachlot:
  • CellAttachment:TestedforABIlitytopromoteattachmentofratPC-12pheochromocytomacells.

Effectsofethanolonglycinergicsynapticcurrentsinmousespinalcordneurons
Mariqueo,T.A.,Agurto,A.,Munoz,B.,SanMartin,L.,Coronado,C.,Fernandez-Perez,E.J.,Murath,P.,Sanchez,A.,Homanics,G.E.andAguayo,L.G.
JNeurophysiol,May2014;111:1940–1948.
http://jn.physiology.org/cgi/content/abstract/111/10/1940

NAD+andSIRT3controlmicrotubuledynamicsandreducesusceptibilitytoantimicrotubuleagents
WilliamT.Harkcom,AnandaK.Ghosh,MatthewS.Sung,AlexandreMatov,KevinD.Brown,ParaskeviGiannakakou,andSamieR.Jaffrey
PNAS,Jun2014;111:E2443–E2452.
http://www.pnas.org/cgi/content/abstract/111/24/E2443

JAZ(Znf346),aSIRT1-interactingProtein,ProtectsNeuronsbyStimulatingp21(WAF/CIP1)ProteinExpression
SathiMallickandSantoshR.D’Mello
J.Biol.Chem.,Dec2014;289:35409–35420.
http://www.jbc.org/cgi/content/abstract/289/51/35409

“DifferentialPotencyof2,6-DimethylcyclohexanolIsomersforPositiveModulationofGABAAReceptorCurrents”
“LuvanaChowdhury,CelineJ.Croft,ShikhaGoel,NainaZaman,AngelaC.-S.Tai,ErinM.Walch,Kelly
Smith,AlexandraPage,KevinM.Shea,C.DennisHall,D.Jishkariani,GirinathG.Pillai,andAdamC.Hall”
J.Pharmacol.Exp.Ther.,May2016;357:570–579.
http://jpet.aspetjournals.org/cgi/content/abstract/357/3/570

RegulationofNeuronalSurvivalbyNucleophosmin1(NPM1)IsDependentonItsExpressionLevel,SubcellularLocalization,andOligomerizationStatus
JasonA.PfisterandSantoshR.D’Mello
J.Biol.Chem.,Sep2016;291:20787–20797
http://www.jbc.org/cgi/content/abstract/291/39/20787