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Trevigen/Etoposide/4684-096-06/100 μl

Description

Althoughtheentiresignalingpathwaysmediatingetoposide-inducedapoptosisarenotclear,double-strandedandsingle-strandedDNAbreaksinducedbyetoposidehavebeenshowntoactivatethep53activitybyphosphorylation,resultinginanup-regulationofthepro-apoptoticproteinBax.ThetranslocationofBaxtothemitochondriainducestheMPT,theeventthatreleasescytochromecandculminatesinthedeathofthecells.EtoposidealsoblocksthecellcycleinS-phaseandG2-phase;inducesapoptosisinnormalandtumorcelllines;inhibitssynthesisoftheoncoproteinMdm2andinducesapoptosisintumorlinesthatoverexpressMdm2.

PhysicalState:EtoposideisprovidedinDMSO.Thefinalreagentconcentrationis10mM.

 

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