企业QQ
官方微信
ThisELISAbasedassaydetectspoly(ADP-ribose)depositedbyPARP-1ontoimmobilizedhistonesina96-wellformat.Ananti-PARmonoclonaldetectingantibodyfollowedbyadditionofagoatanti-rabbitIgG-HRPsecondaryandacolorimetricHRPsubstrateyieldsrelativeabsorbancethatcorrelateswithPARP-1activity. DuringapoptosisPARP-1iscleavedwitharesultingdropinactivity.Etoposideisincludedasacontrolapoptosisinducer.
FEATURES:
APPLICATIONS:
| Catalog#4684-096-Kincludes: | ||
| CatalogNumber | Description | Qty |
| 4671-096-06 | 10XActivatedDNA | 1 |
| 4684-096-01 | PARP-HSA,10mUnits/µl | 1 |
| 4684-096-02 | 20mMNAD | 1 |
| 4684-096-04 | Anti-PARmonoclonalAntibody | 1 |
| 4684-096-05 | Goatanti-mouseIgG-HRP | 1 |
| 4684-096-06 | 10mMEtoposide | 1 |
| 4684-096-07 | 20XI-PARAssayBuffer | 1 |
| 4684-096-03 | 5XAntibodyDiluent | 1 |
| 4684-096-P | Histone-CoatedNaturalStripWellPlate,I-PAR | 1 |
| 4822-96-08 | TACS-Sapphire | 1 |
“UltravioletRADIationSignalingthroughTLR4/MyD88
ConstrainsDNARepairandPlaysaRoleinCutaneous
Immunosuppression”
ErinHarberts,HuaZhou,RitaFishelevich,JuanLiu,andAnthonyA.Gaspari
J.Immunol.,Apr2015;194:3127–3135.
http://www.jimmunol.org/cgi/content/abstract/194/7/3127
“BokIsNotPro-ApoptoticButSuppressesPolyADP-Ribose
Polymerase-DependentCellDeathPathwaysandProtectsagainst
ExcitotoxicandSeizure-InducedNeuronalInjury”
“BeatriceD’Orsi,TobiasEngel,ShonaPfeiffer,SaheliNandi,ThomasKaufmann,DavidC.Henshall,and
JochenH.M.Prehn”
J.Neurosci.,Apr2016;36:4564–4578
http://www.jneurosci.org/cgi/content/abstract/36/16/4564
NAD+repletionimprovesmusclefunctioninmusculardystrophyandcountersglobalPARylation
“DongryeolRyu,HongboZhang,EduardoR.Ropelle,VincenzoSorrentino,DaviA.G.Mázala,LaurentMouchiroud,PhilipL.Marshall,MatthewD.Campbell,AmirSafiAli,GaryM.Knowels,StéphanieBellemin,ShamaR.Iyer,XuWang,KarimGariani,AnthonyA.Sauve,CarlesCantó,KevinE.Conley,LudivineWalter,RichardM.Lovering,EvaR.Chin,BernardJ.Jasmin,DavidJ.Marcinek,KeirJ.Menzies,andJohanAuwerx”
ScienceTranslationalMedicine,Oct2016;8:361ra139
http://stm.sciencemag.org/cgi/content/abstract/8/361/361ra139
SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn