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ThisELISAbasedassaydetectspoly(ADP-ribose)depositedbyPARP-1ontoimmobilizedhistonesina96-wellformat.Ananti-PARmonoclonaldetectingantibodyfollowedbyadditionofagoatanti-rabbitIgG-HRPsecondaryandacolorimetricHRPsubstrateyieldsrelativeabsorbancethatcorrelateswithPARP-1activity. DuringapoptosisPARP-1iscleavedwitharesultingdropinactivity.Etoposideisincludedasacontrolapoptosisinducer.
FEATURES:
APPLICATIONS:
| Catalog#4684-096-Kincludes: | ||
| CatalogNumber | Description | Qty |
| 4671-096-06 | 10XActivatedDNA | 1 |
| 4684-096-01 | PARP-HSA,10mUnits/µl | 1 |
| 4684-096-02 | 20mMNAD | 1 |
| 4684-096-04 | Anti-PARmonoclonalAntibody | 1 |
| 4684-096-05 | Goatanti-mouseIgG-HRP | 1 |
| 4684-096-06 | 10mMEtoposide | 1 |
| 4684-096-07 | 20XI-PARAssayBuffer | 1 |
| 4684-096-03 | 5XAntibodyDiluent | 1 |
| 4684-096-P | Histone-CoatedNaturalStripWellPlate,I-PAR | 1 |
| 4822-96-08 | TACS-Sapphire | 1 |
“UltravioletRADIationSignalingthroughTLR4/MyD88
ConstrainsDNARepairandPlaysaRoleinCutaneous
Immunosuppression”
ErinHarberts,HuaZhou,RitaFishelevich,JuanLiu,andAnthonyA.Gaspari
J.Immunol.,Apr2015;194:3127–3135.
http://www.jimmunol.org/cgi/content/abstract/194/7/3127
“BokIsNotPro-ApoptoticButSuppressesPolyADP-Ribose
Polymerase-DependentCellDeathPathwaysandProtectsagainst
ExcitotoxicandSeizure-InducedNeuronalInjury”
“BeatriceD’Orsi,TobiasEngel,ShonaPfeiffer,SaheliNandi,ThomasKaufmann,DavidC.Henshall,and
JochenH.M.Prehn”
J.Neurosci.,Apr2016;36:4564–4578
http://www.jneurosci.org/cgi/content/abstract/36/16/4564
NAD+repletionimprovesmusclefunctioninmusculardystrophyandcountersglobalPARylation
“DongryeolRyu,HongboZhang,EduardoR.Ropelle,VincenzoSorrentino,DaviA.G.Mázala,LaurentMouchiroud,PhilipL.Marshall,MatthewD.Campbell,AmirSafiAli,GaryM.Knowels,StéphanieBellemin,ShamaR.Iyer,XuWang,KarimGariani,AnthonyA.Sauve,CarlesCantó,KevinE.Conley,LudivineWalter,RichardM.Lovering,EvaR.Chin,BernardJ.Jasmin,DavidJ.Marcinek,KeirJ.Menzies,andJohanAuwerx”
ScienceTranslationalMedicine,Oct2016;8:361ra139
http://stm.sciencemag.org/cgi/content/abstract/8/361/361ra139
matriks/Shikari® (S-ATP) Anti-Pembrolizumab ELISA w/confirmation/PEM-QNS-KEY
intactgenomics/Methionine Auxotrophic LBA4404 | ig® | Intact Genomics/15x50μl/1076-15
goprolytix/Proteolytic Activation of Prothrombin/1 mg, 200 µg/BCT-DFP
novateinbio/Dynorphin A ( 13-17 ) Porcine Peptide/5mg/PE-54024_5mg
matriks/Shikari® (Q-ETA) Etanercept ELISA/ETA-FD-ENB
intactgenomics/Agrobacterium ElectroComponent Combo Pack | Intact Genomics/12x50µl/1290-24
Jackson/Peroxidase AffiniPure Goat Anti-Mouse IgG (H+L)/2.0 ml/115-035-003
Jackson/Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L)/2.0 ml/111-035-003
Qubit™ dsDNA BR Assay Kit Q32853现货
Invitrogen Q32855 Qubit™ RNA HS Assay Kit现货促销
LUCK-1G,D-Luciferin, Potassium Salt 钾盐现货
GoldBio/D-Luciferin, Sodium Salt (Proven and Published™)/LUCNA-1G/1 g