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SBI/Anti-TSG101 Antibody (rabbit anti-human, mouse, rat) with goat anti-rabbit HRP secondary antibody/EXOAB-TSG101-1/25188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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SBI/Anti-TSG101 Antibody (rabbit anti-human, mouse, rat) with goat anti-rabbit HRP secondary antibody/EXOAB-TSG101-1/25

Overview:

Confirmexosomeisolationwithconfidence

Whenyou’rereadytoconfirmthatyou’veisolatedexosomes,stickwiththeexosomeexpertsatSBI.Ourpolyclonalrabbitanti-TSG101antibodiesrecognizethehuman,mouse,andratformsoftheprotein,andareagreatchoiceforgeneralexosomedetection,especiallywhenusedinconjunctionwithantibodiesforotherexosomeMarkerssuchasthetetraspaninsCD9,CD63,andCD81,aswellastheheatshockproteinHSP70(ifyouhaveanyquestionsaboutthebestantibodiestouseforyourexosomeproject,we’rereadytohelp!Justemailusattech@systembio.com).And,likeallofourantibodies,ouranti-TSG101antibodyandtheincludedgoatanti-rabbitHRPsecondaryantibodiesarerigorouslyvalidatedforexosomedetectionviaWesternblotting.

  • Rigorouslyvalidated
  • Hand-pickedselection
  • Expertexosomesupport

Choosetherightantibodyforyourexosomedetectionneeds

Cat.#AntibodytoNotesLinktoExoCartaentryforthehumanprotein
Generalexosomemarkers
EXOAB-CD9A-1CD9TetraspaninExoCarta_928
EXOAB-CD63A-1CD63TetraspaninExoCarta_967
EXOAB-CD81A-1CD81TetraspaninExoCarta_93185
EXOAB-Hsp70A-1HSP70ExoCarta_3303,ExoCarta_3304,ExoCarta_3306,ExoCarta_3308,ExoCarta3310,ExoCarta_3311
EXOAB-ALIX-1ALIXProgrammedcelldeath6interactingprotein(PDCD6IP)ExoCarta_10015
EXOAB-ANXA5-1ANXA5-1AnnexinA5ExoCarta_308
EXOAB-TSG101-1TSG101Tumorsusceptibilitygene101ExoCarta_7251
EXOAB-FLOT1-1FLOT-1Flotillin-1ExoCarta_10211
Tissue-specificexosomemarkers
EXOAB-CXCR4-1CXCR4SpecificforB-cells,T-cells,andplateletsExoCarta_7852
EXOAB-EPCAM1-1EpCamSpecificforcancer-derivedexosomesExoCarta_4072
EXOAB-VMTN-1VimentinSpecificformesenchymalstemcellsExoCarta_7431

SupportingData:

SeeisolationdatausingExo-FLOWIPKits

Forthesestudies,eitherhumanserumorHEK293exosomesconcentratedfromcellculturemediausingExoQuick-TCwereaddedtotheantibody-coupledmagneticbeads.Afterwashing,exosomeswereelutedandrecoveryestimatedusingastandardBCAproteinassay.

Western Blot showing selective immunopurification using Exo-Flow96 IP Kits

Figure1.CD63andCD9,twoexosomemarkers,arereADIlydetectedinsamplespurifiedusingExo-FlowKits.Approximately1µgofproteinwasloadedperwellona4-20%gradientproteinPAGE.TheproteinswereseparatedandtransferredtonitrocellulosemembranesforWesternblotanalysis.Theblotswereprobedwitheitherwithanti-CD63oranti-CD9antibodiestodetecttheexosomeproteinmarkers.

NanoSight analysis shows Exo-Flow Kits deliver expected exosome-sized particles and good yieldsNanoSight analysis shows Exo-Flow Kits deliver expected exosome-sized particles

Figure2.NanoSightanalysisshowsExo-FlowIPKitsdelivergoodyieldsofparticleswhosesizesareconsistentwithexosomes.

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