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Mammalian cell culture in a serum free medium requires an iron transporter such as transferrin. Human transferrin is a 77 kDa beta-glycoprotein with a range of phenotypes based upon the number of sialic acid residues. Each molecule of transferrin specifically binds two Fe3+ molecules.
The iron content can be adjusted to give near 100% saturation to yield holo-transferrin (Iron 1200-1600 ug/gm) or depleted to give near zero iron bound to yield apo-transferrin (Iron <50ug/gm protein) or the intermediate- sidero-transferrin (Iron 300-1000ug/gm protein).
The secondary role for transferrin in culture media is to bind endogenous metal iron which may cause cell toxicity.
SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn