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ABTbeads/Sepadextran™-25 Superfine/SD25SF-500-inquire

Sepadextran™isabeadedgelfiltrationmediumpreparedbycrosslinkingdextrananditissuppliedinitsdryform.Sepadextran™-25canbeusedforproteinandnucleicacidpurificationsbeingtheexclusionlimit5kDforproteinsand10basesfornucleicacids.Desalting(beforeIEXandafterHICorAffinityChromatography)&bufferexchange(betweendifferentchromatographysteps)areotheruses.

SuperFinegradeareforpreparativeseparationsandroutinelaboratorywork.

ProductNameSepadextran™-25Superfine
Cat.No.SD25SF-X
MatrixCrosslinkeddextran
WaterRegain2.15-2.25ml/g
Swelling4-6ml/g
DryParticleSize20-50µm(>80%)
WetParticleSize35-90µm
MaximumOperatingPressureGenerallyobeysDarcy’sLaw:U=KoΔP/LWhere:U=linearflowrate(cm/h).ΔP=pressuredropovergelbead(cmH2O).L=bedhigh(cm).Ko=9
FractionationRange1.5kDforglobularproteins,100-5,000Dfordextrans
pHStABIlity2.0to13
Autoclavable121˚C,pH7.0(30minutes)
ChemicalStabilityAllcommonlyusedbuffers,including:0.1MNaOH;0.01MHCl;1Maceticacid;8Murea;6MguanidineHCl;1%SDS,24%Ethanol;30%Propanol;30%Acetonitrile
StorageTemperatureAmbient
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