DNAzol®isacompleteandreadytousereagentfortheisolationofgenomicorviralDNAfromsolidandliquidsamplesofhuman,animalandplantorigin.TheDNAzolprocedureisbasedontheuseofanovelguanidine–detergentlysingsolutionthathydrolyzesRNAandpromotestheselectiveprecipitationofDNAfromthecelllysate(1,2).ThisadvancedDNAisolationmethodcombinesreliABIlityandefficiencywiththesimplicityofafastisolationprotocol.DNAzol®isanon-toxicsolutionandtheproceduredoesnotrequiretheuseofphenols.DNAcanbeobtainedfromalargenumberofsamplesofsmallorlargevolume;50mlofDNAzol®issufficienttoprocess50samples,eachwith25–50mgoftissue.GenomicDNAisolationcanbecompletedin10–30minuteswithDNArecoveryof70–100%.IsolatedDNAcanbeusedforSouthernanalysis,dotblothybridization,molecularcloningandpolymerasechainreaction.Thereare3versionsofDNAzol®,eachdesignedforoptimalisolationefficiencyfromvarioustypesofsamples.
DNAzol®(DN127)
$58.00–$452.00
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DNAzol®isforusewithtissues,cellsandliquidsamples.1.0mlofDNAzolisolatesDNAfrom25–50mgtissueorfromcellpelletscontaining1–3x107cells.
DNAzol®ES(DN128)
$60.00–$476.00
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DNAzol®ESisextrastrengthDNAzolfortheisolationofgenomicDNAfromplants.1.5mlofDNAzol®ESisolatesDNAfrom0.5gofplanttissue.
DNAzol®BD(DN129)
$60.00–$476.00
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DNAzol®BDisspecificallydesignedfortheisolationofgenomicorviralDNAfromwholeblood.1.0mlofDNAzol®BDisolatesDNAfrom0.5mlofwholeblood.
References
USPatent5,945,515.
- Chomczynski,P.,Mackey,K.,Drews,R.,andWilfinger,W.DNAzol®:AreagentfortherapidisolationofgenomicDNA.BioTechniques.22:550-553.1997.
- Mackey,K.,Williams,P.,Seim,S.andChomczynski,P.TheuseofDNAzol®fortherapidisolationofgenomicDNAforwholeblood.Biomed.Products.Supplement:13-15.1996.