IntheNeuroProbeAP48chemotaxischamber,chemoattractantsaddedtothelowerwellsactoncellsinasUSPensionaddedtotheupperwells.Afilterseparatestheupperandlowerwells.Afterincubationthemigratedadherentcellsontheundersideofthefilterarestainedandcounted.
TheAP48consistsofprecision-machinedacrylictopandbottomplates,assemblyhardware,andasiliconegasket.Thebottomplatehas48wells,eachwith25µLvolumeandadiameterof3.2mm.Correspondingholesinthetopplateformtheupperwellswhenthechamberisassembled.Asingle25x80mmpieceoffiltermembraneisplacedbetweenthetopandbottomplates,andagasketispositionedoverthefiltertocreateaseal.Narrowraisedrimsaroundmatingsurfacesoftopandbottomwellsenhancetheseal.
Beforethemigratedcellscanbestainedandcounted,thenonmigratedcellsmustbewipedoffthetopsideofthefilter.An accessorypack(stock#P48AP)includesinstrumentsforthispurpose.
TheAP48isdesignedforusewithpolycarbonateandcellulosenitratefilters.Pleasenotethatwealsosellthe AC48,a48-wellchamberspeciallydesignedforusewithcellulosenitratefilters
Accompanyingeachchamberisan AP48protocol foritsuseandcare.
Stock # | Exposedfilterarea | Bottomwellvolume | Topwellvolume | Filtersused | |
---|---|---|---|---|---|
AP48 | 8mm2 | 25µL | 50µL | 25x80mmpolycarbonate 25x80mmcellulosenitrate |
Please contactus toorderreplacementhardware,orifyouhaveanyquestions.
Weight | 0.4375lbs |
---|---|
Dimensions | 12x9x2in |
PlateSize | 48-wellplate |
SouthernBiotech/Goat Anti-Human Lambda-Alexa Fluor® 555/2070-32/1.0 mg
vectorlabs/Biotinylated Aleuria Aurantia Lectin (AAL)/B-1395/1 mg
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Mouse IgG)/PK-6102/1 kit
vectorlabs/VECTASTAIN® Elite® ABC-HRP Kit (Peroxidase, Universal)/PK-6200/1 kit
vectorlabs/Unconjugated Musa Paradisiaca (Banana) Lectin (BanLec)/L-1410/5 mg
GiottoBiotech/Calmodulin N60D/5 mg/G02CLM60cn