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Cosmo/Di-ubiquitin (K63-linked) [untagged]/10 µg/UBI-60-0107-010188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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Cosmo/Di-ubiquitin (K63-linked) [untagged]/10 µg/UBI-60-0107-010

Species Reactivity: humanSource: enzyme catalysedFormula: 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol Concentration: 0.5 mg/mlMolecular Weight: ~17.1 kDaProtein Sequence: Accession number: P62987. For full protein sequence information download the Certificate of Analysis pdf.Quality Control Protein Identification: The linkage type (K63) was confirmed by tandem mass spectrometry.Quality Control Activity: Di-ubiquitin cleavage assay: The capacity of the di-ubiquitin substrate to be cleaved was tested using a promiscuous- with respect to ubiquitin linkage specificity- deubiquitylase (GST-USP2). Incubation of the di-ubiquitin for 1 hour at 37 °C was compared either in the absence (Lane 2) or presence (Lane 3) of GST-USP2. The reaction products were compared alongside two control samples containing either mono-ubiquitin (Lane 4) or GST-USP2 (Lane 5) only. Cleavage of the di-ubiquitin and generation of mono-ubiquitin was determined by running reactions on a 4-12%­SDS-PAGE gel and staining with InstantBlueâ„¢ (Lane 1; molecular weight markers).
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