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VitaNavi/Direct Cell PCR Kit/VN431DKGC

DirectCellPCRKit containsallthereagentsneededforquickamplificationofgenomicDNAfromcells.Thiskitcanbealsoappliedtodirectamplificationofexogenoustargetgenes,suchasvirus,bacteriaandparasitesfromcellculture.Thekitisoptimizedwithouruniqueinhibitor-resistantDNApolymerasesandbufferforbestperformance. Thereisthe choiceofaninertgreendye inthemix,usedfordirectgelloADIng. ThePCRproductcanbedirectlyloadedontoagarosegelwithoutadditionofloadingdyeforelectrophoresis.

GCversionworksforGC-richandcomplextarget.

Highlight

  • Timesaving: Noneedfor DNAextrationorpre-lysisofsampleasothersimilarcommercialkits
  • Convenientandflexibility: Onlyneedaddingenzyme,primersandsamplein2Xmix.Theenzymeispackedinaseparatedvial.Thecustomersareabletotitratetheamountofenzymetofindthebestonefortheirtargets.Thewholemastermixincludingtheenzymeinsideisavailableaswell(Clickhere)
  • Highinhibitor-resistance: Resistancetoinhibitorsinbothcellsandmedia
  • Specificity: GCversionworksfor>65%GCcontenttarget,increasesspecificity
  • Simplyanalysis:Directlyloadingproductsforelectrophoresis

Applications

  • Genotyping
  • Colonyscreening
  • Mutationdetection
  • Pathogendetection

DataSheet:DirectCellPCRKit

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