Cdc42 Protein

货号：	10107
CAS号：	10107
供应商：	10107
数量：	30000
英文名：	Cdc42Protein
保质期：	一年
保存条件：	-20℃
规格：	100μl

Cdc42Protein[10107]
[100µg]

CatalogNumber: 10107

ProductName: Cdc42Protein

Synonyms: Celldivisioncycle42,G25K,CDC42Hs

Source: Human,recombinantfulllength,His6-tag

ExpressionHost: E.coli

MolecularWeight: 21kDa

Purity: >95%bySDS-PAGE

Introduction: SmallGTPasesareasuper-familyofcellularsignalingregulators.Cdc42belongstotheRhosub-familyofGTPasesthatregulatecellmotility,celldivision,andgenetranscription.GTPbindingincreasestheactivityofCdc42,andthehydrolysisofGTPtoGDPrendersitinactive.GTPhydrolysisisaidedbyGTPaseactivatingproteins(GAPs),whileexchangeofGDPforGTPisfacilitatedbyguaninenucleotideexchangefactors(GEFs).

AminoAcidSequence (1-191)

MQTIKCVVVGDGAVGKTCLLISYTTNKFPSEYVPTVFDNYAVTVMIGGEPYTLGLFDTAGQEDYDRL
RPLSYPQTDVFLVCFSVVSPSSFENVKEKWVPEITHHCPKTPFLLVGTQIDLRDDPSTIEKLAKNKQ
KPITPETAEKLARDLKAVKYVECSALTQKGLKNVFDEAILAALEPPEPKKSRRCVLL

Properties

PhysicalAppearance(form): Dissolvedin20mMTris-HCl,pH8.0,150mMNaCl.

PhysicalAppearance(form): Whiteorclear

Concentration: 1mg/mL

Storage: -80°C

PreparationInstructions:
Centrifugethevialbeforeopenthecapandreconstituteinwater.Addingof10mMβ-mercaptoethanolor1mMDTTintothesolutiontoprotecttheproteinisrecommendedandusingofnon-ionicdetergentssuchasn-Dodecylβ-D-maltoside(DoDM)orpolyethylenedetergents(e.g.C12E10)alsohelptostABIlizetheprotein.Avoidrepeatedfreezingandthawingafterreconstitution.ThepurityofHis-taggedCdc42wasdeterminedbySDS-PAGEandCoomassieBrilliantBlueStaining.

References:
1.Garrett,W.S..etal.,Cell102:325-334,2000.
2.Irie,F.etal.,NatureNeurosci.5:1117-1118,2002.
3.Kawasaki,Y.etal.,Oncogene26:7620-7627,2007.
4.Manser,E.etal.,Nature363:364-367,1993.
5.Musch,A.etal.,EMBOJ.20:2171-2179,2001.
6.Nalbant,P.etal.,Science305:1615-1619,2004.
7.Shen,Y.etal.,Dev.Cell14:342-353,2008.
8.Wu,W.J.etal.,Nature405:800-804,2000.
9.Wu,X.etal.,GenesDev.20:571-585,2006.
10.Zheng,Y.etal.,J.Biol.Chem.271:33169-33172,1996.

Datasheet:

Publications:

1. VibrioparahaemolyticusEffectorProteinsSuppressInflammasomeActivationbyInterferingwithHostAutophagySignaling
PLoSPathog.2013Jan;9(1):e1003142

2. Interferongammainducesactinpolymerization,Rac1activationanddownregulatesphagocytosisinhumanmonocyticcells
Cytokine.2012Jan;57(1):158-68

3. Invadopodiaandrolling-typemotilityarespecificfeaturesofhighlyinvasivep190bcr-ablleukemiccells
EuropeanJournalofCellBIOLOGyVolume91,Issues11–12,November–December2012,Pages978–987

4. EpithelialjunctionformationrequiresconfinementofCdc42activitybyanovelSH3BP1complex
JCellBiol.2012Aug20;198(4):677-93

5. PolarizedmigrationoflymphaticendothelialcellsiscriticallydependentonpodoplaninregulationofCdc42
AmJPhysiolLungCellMolPhysiol.2011Jan;300(1):L32-42

6. TheAarskog-ScottSyndromeProteinFgd1RegulatesPodosomeFormationandExtracellularMatrixRemodelinginTransformingGrowthFactorbeta-StimulatedAorticEndothelialCells
MolCellBiol.2011Nov;31(22):4430-41

7. MMP-9andCXCL8/IL-8ArePotentialTherapeuticTargetsinEpidermolysisBullosaSimplex
PLoSOne.2013Jul19;8(7):e70123

8. p21-ActivatedKinase(PAK)RegulatesCytoskeletalReorganizationandDirectionalMigrationinHumanNeutrophils
PLoSOne.2013Sep3;8(9):e73063

9. TheCdc42GuanineNucleotideExchangeFactorFGD6CoordinatesCellPolarityandEndosomalMembraneRecyclinginOsteoclasts
JBiolChem.2014Jun27;289(26):18347-59

10. Cdc42InhibitorandUsesThereof
UnitedStatesPatentApplication20140194451

11. Theα4NicotinicReceptorPromotesCD4+T-CellProliferationandaHelperT-CellImmuneResponse
MolecularPharmacologyJanuary2014vol.85no.150-61

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Cdc42 Protein