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ProductDescription
Rab7belongstotheRabfamilyofsmallGTPases.Rab7islocatedinthelateendosome,lysosome,cytoplasmicvesicle,phagosomeandmelanosome.Itisinvolvedinthelateendocytictransportandcontributestothematurationofphagosomes. CurrentlythereisnodirectassaytomeasuretheactivationofRab7GTPases. NewEastBiosciencesRab7ActivationAssayKitisbasedontheconfiguration-specificmonoclonalantibodythatspecificallyrecognizesRab7-GTP,butnotRab7-GDP.Giventhehighaffinityofmonoclonalantibodiestotheirantigens,theactivationassaycouldbeperformedinashorttime.Thisassayprovidesthereliableresultswithconsistentreproducibility. Theseanti-Rab7-GTPmonoclonalantibodiescanalsobeusedtomonitortheactivationofRab7incellsandintissuesbyimmunohistochemistry. NewEastBiosciencesRab7ActivationAssayKitprovidesasimpleandfastmethodtomonitortheactivationofRab7.Eachkitprovidessufficientquantitiestoperform20assays. AssayPrincipleNewEastBiosciencesRab7ActivationAssayKitbasesontheconfiguration-specificanti-Rab7-GTPmonoclonalantibodytomeasuretheactiveRab7-GTPlevels,eitherfromcellextractsorfrominvitroGTPγSloADIngRab7activationassays.Briefly,anti-activeRab7mousemonoclonalantibodywillbeincubatedwithcelllysatescontainingRab7-GTP.TheboundactiveRab7willthenbepulleddownbyproteinA/Gagarose.TheprecipitatedactiveRab7willbedetectedbyimmunoblotanalysisusinganti-Rab7rabbitpolyclonalantibody. ProductManual
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