Cdc42 activation assay kit 活性蛋白检测试剂盒

货号：	80701
数量：	大量
英文名：	Cdc42ActivationAssayKit
供应商：	NewEastbio
保质期：	24个月
保存条件：	-20℃冻存
规格：	20T

Cdc42ActivationAssayKit[80701]
[20assays]

ProductHighlight

Configuration-specificmonoclonalantibodythatrecognizesCdc42-GTP,butnotCdc42-GDP
TheonlyavailableCdc42activationassaykitdirectlymeasuresthelevelofCdc42-GTP
ProvidingreliableresultswithconsistentreproducibilitythanotherCdc42activationassaykitsbasedoncertaindownstreamsignalingpathways
Providingshorterexperimentaltime
CouldbeusedtomonitortheactivationofCdc42incellsandintissuesbyimmunohistochemistry

ProductDescription

SmallGTPasesareasuper-familyofcellularsignalingregulators.Cdc42belongstotheRhosub-familyofGTPasesthatregulatecellmotility,celldivision,andgenetranscription.GTPbindingincreasestheactivityofCdc42,andthehydrolysisofGTPtoGDPrendersitinactive.

CurrentlytheactivationofCdc42proteinsisassayedwiththebindingofGTP-boundCdc42tothep21-bindingdomain(PBD)ofp21-activatedproteinkinase(PAK).Thismethodisbasedontheobservationthattheactive,GTP-boundCdc42(andRac)couldbindtothePBDofPAK.However,thereproducibilityofthismethodispoor.ThisispartiallyduetotherelativelyquickhydrolysisofGTPtoGDPduringtheassayprocedure,andthelowbindingaffinityofPBDtoCdc42-GTP.

NewEastBiosciencesCdc42ActivationAssayKitisbasedontheconfiguration-specificmonoclonalantibodythatspecificallyrecognizesCdc42-GTP,butnotCdc42-GDP.Giventhehighaffinityofmonoclonalantibodiestotheirantigens,theactivationassaycouldbeperformedinamuchshortertime.Thisassayprovidesthereliableresultswithconsistentreproducibility.

Theseanti-Cdc42-GTPmonoclonalantibodycanalsobeusedtomonitortheactivationofCdc42incellsandintissuesbyimmunohistochemistry.

NewEastBiosciencesCdc42ActivationAssayKitprovidesasimpleandfastmethodtomonitortheactivationofCdc42.Eachkitprovidessufficientquantitiestoperform20assays.

AssayPrinciple

NewEastBiosciencesCdc42ActivationAssayKitbasesontheconfiguration-specificanti-Cdc42-GTPmonoclonalantibodytomeasuretheactiveCdc42-GTPlevels,eitherfromcellextractsorfrominvitroGTPγSloADIngCdc42activationassays.Briefly,anti-activeCdc42mousemonoclonalantibodywillbeincubatedwithcelllysatescontainingCdc42-GTP.TheboundactiveCdc42willthenbepulleddownbyproteinA/Gagarose.TheprecipitatedactiveCdc42willbedetectedbyimmunoblotanalysisusinganti-Cdc42rabbitpolyclonalantibody.

ProductManual
ProtocolforIF/IHC


RelatedProducts
Catalog#	Name	Size
80701	Cdc42ActivationAssayKit	20assays
26905	ActiveCdc42-GTPMonoclonalAntibody	30μL
26008	Anti-Cdc42MouseMonoclonalAntibody	100μL
21010	Anti-Cdc42RabbitPolyclonalAntibody	100μL

Publications:

1. VibrioparahaemolyticusEffectorProteinsSuppressInflammasomeActivationbyInterferingwithHostAutophagySignaling
PLoSPathog.2013Jan;9(1):e1003142

2. Interferongammainducesactinpolymerization,Rac1activationanddownregulatesphagocytosisinhumanmonocyticcells
Cytokine.2012Jan;57(1):158-68

3. Invadopodiaandrolling-typemotilityarespecificfeaturesofhighlyinvasivep190bcr-ablleukemiccells
EuropeanJournalofCellBIOLOGyVolume91,Issues11–12,November–December2012,Pages978–987

4. EpithelialjunctionformationrequiresconfinementofCdc42activitybyanovelSH3BP1complex
JCellBiol.2012Aug20;198(4):677-93

5. PolarizedmigrationoflymphaticendothelialcellsiscriticallydependentonpodoplaninregulationofCdc42
AmJPhysiolLungCellMolPhysiol.2011Jan;300(1):L32-42

6. TheAarskog-ScottSyndromeProteinFgd1RegulatesPodosomeFormationandExtracellularMatrixRemodelinginTransformingGrowthFactorbeta-StimulatedAorticEndothelialCells
MolCellBiol.2011Nov;31(22):4430-41

7. MMP-9andCXCL8/IL-8ArePotentialTherapeuticTargetsinEpidermolysisBullosaSimplex
PLoSOne.2013Jul19;8(7):e70123

8. p21-ActivatedKinase(PAK)RegulatesCytoskeletalReorganizationandDirectionalMigrationinHumanNeutrophils
PLoSOne.2013Sep3;8(9):e73063

9. TheCdc42GuanineNucleotideExchangeFactorFGD6CoordinatesCellPolarityandEndosomalMembraneRecyclinginOsteoclasts
JBiolChem.2014Jun27;289(26):18347-59

10. Cdc42InhibitorandUsesThereof
UnitedStatesPatentApplication20140194451

11. Theα4NicotinicReceptorPromotesCD4+T-CellProliferationandaHelperT-CellImmuneResponse
MolecularPharmacologyJanuary2014vol.85no.150-61

温馨提示：不可用于临床治疗。

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Cdc42 activation assay kit 活性蛋白检测试剂盒

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ProductDescription

AssayPrinciple