Arf1 Protein Δ17 Q71L mutant

货号：	10123
CAS号：	10123
供应商：	NewEastBio
数量：	30000
英文名：	Arf1ProteinΔ17Q71Lmutant
保质期：	一年
保存条件：	-20℃
规格：	10μl

Arf1ProteinΔ17Q71Lmutant[10123]
[10µg]

CatalogNumber: 10123

ProductName: Arf1ProteinΔ17Q71Lmutant

Synonyms: ADP-ribosylationfactor1

Source: Humanrecombinant,His6-tag

ExpressionHost: E.coli

MolecularWeight: 21kDa

Purity: >95%bySDS-PAGE

Introduction: Arf1isamemberoftheARFsuper-family.ARFgenesencodesmallGTPasesthatincreasetheADP-ribosyltransferaseactivityofcholeratoxinandarecriticalforvesiculartraffickingasactivatorsofphospholipaseD.Arf1proteinislocalizedtotheGolgiapparatusandhasacentralroleinintra-Golgitransport.

AminoAcidSequence (1-181,Δ17,Q71L)

MGNIFANLFKGLFGKK-MRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWD
VGGLDKIRPLWRHYFQNTQGLIFVVDSNDRERVNEAREELMRMLAEDELRDAVLLVFANKQDLPNAM
NAAEITDKLGLHSLRHRNWYIQATCATSGDGLYEGLDWLSNQLRNQK

Properties

PhysicalAppearance(form): Dissolvedin20mMTris-HCl,pH8.0,150mMNaCl.

PhysicalAppearance(form): Whiteorclear

Concentration: 1mg/mL

Storage: -80°C

PreparationInstructions:
Centrifugethevialbeforeopenthecapandreconstituteinwater.Addingof10mMβ-mercaptoethanolor1mMDTTintothesolutiontoprotecttheproteinisrecommendedandusingofnon-ionicdetergentssuchasn-Dodecylβ-D-maltoside(DoDM)orpolyethylenedetergents(e.g.C12E10)alsohelptostABIlizetheprotein.Avoidrepeatedfreezingandthawingafterreconstitution.ThepurityofHis-taggedArf1Δ17Q71LwasdeterminedbySDS-PAGEandCoomassieBrilliantBlueStaining

References:
1.Amor,J.C.etal.,Nature372:704-708,1994.
2.Bobak,D.A.etal.,Proc.Nat.Acad.Sci.86:6101-6105,1989.
3.Hirai,M.etal.,Genomics34:263-265,1996.
4.Kumari,S.etal.,CellBiol.10:30-41,2008.
5.Lee,C.-M.etal.,J.Biol.Chem.267:9028-9034,1992.
6.Mossessova,E.etal.,Cell92:415-423,1998.
7.Peng,Z.G.etal.,Biofactors2:45-49,1989.
8.Presley,J.F.etal.,Nature417:187-193,2002.
9.Renault,L.etal.,Nature426:525-530,2003.

Datasheet:

温馨提示：不可用于临床治疗。

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Arf1 Protein Δ17 Q71L mutant