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| 货号： | 10101 |
| CAS号： | 10101 |
| 供应商： | NewEastBio |
| 数量： | 30000 |
| 英文名： | Rac1Protein |
| 保质期： | 一年 |
| 保存条件： | -20℃ |
| 规格： | 100μl |
| CatalogNumber: 10101 |
| ProductName: Rac1Protein |
| Synonyms: Ras-relatedC3botulinumtoxinsubstrate1,Rac-1,p21-Rac1,MIG5,TC-25 |
| Source: Human,recombinantfulllength,His6-tag |
| ExpressionHost: E.coli |
| MolecularWeight: 21kDa |
| Purity: >95%bySDS-PAGE |
| Introduction: SmallGTPasesareasuper-familyofcellularsignalingregulators.RacbelongstotheRhosub-familyofGTPasesthatregulatecellmotility,celldivision,andgenetranscription.GTPbindingincreasestheactivityofRac,andthehydrolysisofGTPtoGDPrendersitinactive.GTPhydrolysisisaidedbyGTPaseactivatingproteins(GAPs),whileexchangeofGDPforGTPisfacilitatedbyguaninenucleotideexchangefactors(GEFs). |
| AminoAcidSequence (1-192) |
| MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVMVDGKPVNLGLWDTAGQEDYDRL RPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHHCPNTPIILVGTKLDLRDDKDTIEKLKEKKL TPITYPQGLAMAKEIGAVKYLECSALTQRGLKTVFDEAIRAVLCPPPVKKRKRKCLLL |
| Properties |
| PhysicalAppearance(form): Dissolvedin20mMTris-HCl,pH8.0,150mMNaCl. |
| PhysicalAppearance(form): Whiteorclear |
| Concentration: 1mg/mL |
| Storage: -80°C |
| PreparationInstructions: Centrifugethevialbeforeopenthecapandreconstituteinwater.Addingof10mMβ-mercaptoethanolor1mMDTTintothesolutiontoprotecttheproteinisrecommendedandusingofnon-ionicdetergentssuchasn-Dodecylβ-D-maltoside(DoDM)orpolyethylenedetergents(e.g.C12E10)alsohelptostABIlizetheprotein.Avoidrepeatedfreezingandthawingafterreconstitution.ThepurityofHis-taggedRac1wasdeterminedbySDS-PAGEandCoomassieBrilliantBlueStaining. |
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| References: 1.AznarBenitah,S.etal.,Science309:933-935,2005. 2.Chuang,Y.etal.,CancerRes.64:8271-8275,2004. 3.Halet,G.etal.,Dev.Cell12:309-317,2007. 4.Machacek,M.etal.,Nature461:99-103,2009.5.Matos,P.etal.,Biochem.Biophys.Res.Commun.277:741-751,2000. 6.Moore,K.A.etal.,Biochem.J.326:17-20,1997.7.Walmsley,M.J.etal.,Science302:459-462,2003. 8.Wu,Y.I.etal.,Nature461:104-108,2009. |
Datasheet:  |
| Publications: |
| 1. Phosphorylationofα-tubulinbyproteinkinaseCstimulatesmicrotubuledynamicsinhumanbreastcells CytoskeletonVolume71,Issue4,pages257–272,April2014 |
| 2. ImmunofluorescenceandConfocalMicroscopyofNeutrophils NeutrophilMethodsandProtocolsMethodsinMolecularBIOLOGyVolume1124,2014,pp251-268 |
| 3. Alinkbetweenthenuclear-localizedsrGAP3andtheSWI/SNFchromatinremodelerBrg1 MolecularandCellularNeuroscienceVolume60,May2014,Pages10–25 |
| 4. SHP2PhosphatasePromotesMastCellChemotaxistowardStemCellFactorviaEnhancingActivationoftheLyn/Vav/RacSignalingAxis TheJournalofImmunologyApril14,20141301155 |
| 5. ROBO1,atumorsuppressorandcriticalmolecularbarrierforlocalizedtumorcellstoacquireinvasivephenotype:StudyinAfrican-AmericanandCaucasianprostatecancermodels InternationalJournalofCancerVolume135,Issue11,pages2493–2506,1December2014 |
| 6. TranscriptionfactorsNRF2andnf-kbarecoordinatedeffectorsoftheRHOfamily,GTPbindingproteinrac1duringinflammation JBiolChem.2014May30;289(22):15244-58 |
| 7. CanonicalandNon-canonicalG-proteinSignalingHelpsCoordinateActinDynamicstoPromoteMacrophagePhagocytosisofZymosan MolCellBiol.2014Nov15;34(22):4186-99 |
| 8. p53DownRegulatesPDGF-InducedFormationofCircularDorsalRufflesinRatAorticSmoothMuscleCells PLoSOne.2014Sep23;9(9):e108257 |
| 9. LPA-mediatedmigrationofovariancancercellsinvolvestranslocalizationofGαi2toinvadopodiaandassociationwithSrcandβ-pix CancerLett.2015Jan28;356(2PtB):382-91 |
| 10. cAMPcontrolstherestorationofendothelialbarrierfunctionafterthrombin-inducedhyperpermeabilityviaRac1activation PhysiologicalReportsPublished24October2014Vol.2no.e12175DOI:10.14814/phy2.12175 |
| 11. CisplatinatSub-toxicLevelsMediatesIntegrinSwitchinLungCancerCells AnticancerResearchDecember2014vol.34no.127111-7117 |
| 12. Semaphorin-3FsuppressesthestemnessofcolorectalcancercellsbyinactivatingRac1 Cancerletters.March1,2015Volume358,Issue1,Pages76–84. |
| 13. GeometrysensingthroughPOR1regulatesRac1activitycontrollingearlyosteoblastdifferentiationinresponsetonanofiberdiameter IntegrBiol(Camb).2014Dec24 |
| 14. InhibitionofRac1ActivityintheHippocampusImpairstheForgettingofContextualFearMemory MolNeurobiol.2015Jan24 |