ACID PICRO MALLORY

4. Stain in Carazzi Haemalum 3 minutes 5. Wash in water 6. Blue in Scotts tap water 7. Wash in water 8. Rinse in 95% alcohol 9. Stain in Picro-orange 2 mins 10. Wash in water until only red blood cells are yellow 11. Stain in acid fuchsin 5 mins 12. Wash in water 13. Stain in equal parts Picro-orange and 80% alcohol 14. Differentiate in phosphomolybdic acid for 8 mins 15. Wash in water 16. Stain in analine blue, 8 mins 17. Wash in water 18. Dehydrate, clear . Mount sections in DPX 1. 0.2% orange G in saturated alcoholic Picric Acid 2. 1% Acid fuchsin in 3% Trichloroacetic Acid 3. 1% Phosphomolybdic Acid 4. 2% Analine blue W5 in 2% Acetic Acid (soluble Blue) NOTE: No responsibility is assumed by The University of Nottingham or the Queens Medical Centre NHS Trust for any injury and/or damage to persons or property as a matter of products liability, negligence or otherwise, or from any use or operation of any methods, products, instructions or ideas contained in the material herein. It is the users responsibilty to ensure that all procedures are carried out according to appropriate Health and Safety requirements.