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BIELSCHOWSKY METHOD188bio精品生物—专注于实验室精品爆款的电商平台 - 蚂蚁淘旗下精选188款生物医学科研用品
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BIELSCHOWSKY METHOD

SECTIONS8micronparaffinwaxsections.

SOLUTIONS

1.SilverA-20%silvernitrate

2.Reducer(Makefresh)

  • Formaldehyde50ml
  • AbsoluteAlcohol200ml
  • Distilledwater750ml

3.SilverB

  • 20%Silvernitrate20ml
  • 95%Alcohol20ml
  • Addammoniauntilprecipitateformedisredissolved
  • Add10moredropsofammonia
4.5%Sodiumthiosulphate5.0.1%Goldchloride

METHOD

  1. Takesectionstodistilledwater
  2. PlaceinsolutionAfor2-3hours
  3. Washinreducerfor2-5minutes
  4. Washin10%alcoholfor2minutes
  5. PlaceinsolutionBfor1-2minutes
  6. Washinaconstantflowofreducerandleavefor3-5minutes
  7. Washindistilledwater
  8. Tone0.1%goldchloridefor2minutes
  9. Washindistilledwater
  10. Fixin5%sodiumthiosulphatefor2minutes
  11. Washintapwater
  12. Dehydrate,clear.MountsectionsinDPX

RESULTSNeuritesaroundsenileplaques,neurofibrillarytangles-Black.


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