Cat.No.141003 | 50µgspecificantibody,lyophilized.Affinitypurifiedwiththeimmunogen.AlbuminandazidewereaddedforstABIlization.Forreconstitutionadd50µlH2Otogeta1mg/mlsolutioninPBS.Thenaliquotandstoreat-20°Cuntiluse. |
Applications | WB:1:100upto1:1000(APstaining)(seeremarks)IP:nottestedyetICC:1:200upto1:2000galleryIHC:1:200galleryIHC-P/FFPE:1:500galleryELISA:yes(seeremarks) |
Immunogen | RecombinantproteincorrespondingtoAA3569to3769fromratBassoon(UniProtId:O88778) |
Reactivity | Reactswith:rat(O88778),mouse(O88737).Nosignal:chicken.Otherspeciesnottestedyet. |
Specificity | Specificforbassoon. |
Remarks | WB:Duetoitslargesize,bassoonrequiresspecialgel-electrophoresisandWesternblotprotocolsforvisualizationbyimmunoblotting.Excellentresultscanbeobtainedwiththe4-12%TRIS-glycinegrADIentgelsfromanamedorNuPAGE3-8%TRIS-AcetategradientgelsfromInvitrogen. ELISA:Suitableasdetectorantibodyforsandwich-ELISAwithcat.no.141021ascaptureantibody(protocolforsandwich-ELISA). |
Datasheet | 141_003.pdf |
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