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Synaptic Systems(SYSY)/Bassoon - 141 003/141 003

Cat.No.14100350µgspecificantibody,lyophilized.Affinitypurifiedwiththeimmunogen.AlbuminandazidewereaddedforstABIlization.Forreconstitutionadd50µlH2Otogeta1mg/mlsolutioninPBS.Thenaliquotandstoreat-20°Cuntiluse.
ApplicationsWB:1:100upto1:1000(APstaining)(seeremarks)IP:nottestedyetICC:1:200upto1:2000galleryIHC:1:200galleryIHC-P/FFPE:1:500galleryELISA:yes(seeremarks)
ImmunogenRecombinantproteincorrespondingtoAA3569to3769fromratBassoon(UniProtId:O88778)
ReactivityReactswith:rat(O88778),mouse(O88737).Nosignal:chicken.Otherspeciesnottestedyet.
SpecificitySpecificforbassoon.
Remarks

WB:Duetoitslargesize,bassoonrequiresspecialgel-electrophoresisandWesternblotprotocolsforvisualizationbyimmunoblotting.Excellentresultscanbeobtainedwiththe4-12%TRIS-glycinegrADIentgelsfromanamedorNuPAGE3-8%TRIS-AcetategradientgelsfromInvitrogen.

ELISA:Suitableasdetectorantibodyforsandwich-ELISAwithcat.no.141021ascaptureantibody(protocolforsandwich-ELISA).

Datasheet141_003.pdf
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