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| Product Name | Caspase 8 Substrate 1, chromogenicAc - IETD - pNA |
| Size | 5 mg |
| Catalog # | AS-25258-5 |
| US$ | $108 |
pNA (4-nitroaniline)-derived caspase substrates are widely used for the colorimetric detection of various caspase activities. Cleavage of pNA peptides by caspases generates pNA that is monitored colorimetrically at ~405 nm. pNA has maximum absorption around 408 nm. Caspase-8 substrate with Km = 66 uM; Substrate for granzyme B | |
| Detailed Information |  Datasheet Material Safety Data Sheets (MSDS) |
| Storage | -20°C desiccated and protected from light |
| References | Grutter MG (2000). Caspases: key players in programmed cell death. Curr Opin Struct Biol 10, 649-55; Gastman BR (2001). Apoptosis and its clinical impact. Head Neck 23, 409-25; Grutter MG (2000). Caspases: key players in programmed cell death. Curr Opin Struct Biol 10, 649-55; Stennicke HR and Salvesen GS (1999). Catalytic properties of the caspases. Cell Death Differ 6, 1054-9; Stennicke HR and Salvesen GS (1998). Properties of the caspases. Biochim Biophys Acta 1387, 17-31; Thornberry NA and Lazebnik Y (1998). Caspases: enemies within. Science 281, 1312-6; Talanian RV, et al. (1997). Substrate specificities of caspase family proteases. J Biol Chem 272, 9677-82; Fassy F, et al. (1998). Enzymatic activity of two caspases related to interleukin-1beta-converting enzyme. Eur J Biochem 253, 76-83; Datta R, et al. (1996). Activation of the CPP32 protease in apoptosis induced by 1-?-D-arabinofuranosylcytosine and other DNA-damaging agents. Blood 88, 1936-43; Koeplinger KA, et al. (2000). Caspase 8: an efficient method for large-scale autoactivation of recombinant procaspase 8 by matrix adsorption and characterization of the active enzyme. Protein Expr Purif 18, 378-87. |
| Molecular Weight | 638.6 |
| Ac-IETD-pNA | |
| Sequence(Three-Letter Code) | Ac-Ile - Glu - Thr - Asp-pNA |
| Product Citations | Rocha, R. et al. (2011). Arsenic and fluoride induce neural progenitor cell apoptosis. Toxicol Lett 10.1016/j.toxlet.2011.03.023.Shao, R. et al. (2005). E1A sensitizes cancer cells to TRAIL-induced apoptosis through enhancement of caspase activation. Mol Cancer Res 3, 219.Tang, C-HA. et al. (2005). Synergism of Rana catesbeiana ribonuclease and IFN-γ triggers distinct death machineries in different human cancer cells. FEBS Lett 579, 265. |
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