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| Product Name | Calcein, AM *UltraPure Grade*, 5 mM solution in anhydrous DMSO |
| CAS | 148504-34-1 |
| Size | 200 µL |
| Catalog # | AS-89203 |
| US$ | $186 |
| Purity | ≥95% by HPLC |
Calcein, AM is a cell-permeant and non-fluorescent compound that is widely used for determining cell viability.In live cells the non-fluorescent calcein, AM is hydrolyzed by intracellular esterases into the strongly green fluorescent anion calcein. The fluorescent calcein is well-retained in the cytoplasm in live cells.Hela cells stained with AnaSpecs Calcein AM fluorescent dye (Cat# AS-89201, AS-89202, AS-89203 and AS-89204). MCF-7 cells grown in 8-well slide chamber for 2 days. Cells were washed twice before adding 1 uM Calcein AM (AnaSpec cat# AS-89202). Images acquired every 3 seconds and total elapsed time on video is 12 min.
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| Images |  |
| Detailed Information |  Material Safety Data Sheets (MSDS) |
| Storage | -20°C desiccated and protected from light |
| Molecular Weight | 994.857 |
| Molecular Formula | C46H46N2O23 |
| Spectral Properties | Abs/Em = 494/517 nm |
| Solvent System | DMSO |
| Product Citations | Robinson, M. et al. (2011). Screening of hepatitis C virus inhibitors using genotype 1a HCV replicon cell lines. Curr Prot Microbiol doi: 10.1002/9780471729259.mc1707s22.Delaunay, JL. et al. (2009). A missense mutation in ABCB4 gene involved in progressive familial intrahepatic cholestasis type 3 leads to a folding defect that can be rescued by low temperature. Hepatology 49, 1218.Zhang, K-H. et al. (2009). Ferritin heavy chainmediated iron homeostasis and subsequent increased reactive oxygen species production are essential for epithelial-mesenchymal transition. Cancer Res. 10.1158/0008-5472.CAN-09-0112. |
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