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Boston Biochem/Human SUMO1 Antibody/A-716

Product Details
FAQs
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SummaryData ExamplesPreparation and StorageReconstitution CalculatorBackgroundProduct DatasheetsRelated Research Areas

Human SUMO1 Antibody Summary

Source
Monoclonal Rat IgG2a Clone # 852620
Immunogen
Purified, recombinant human SUMO1Accession # P63165
Formulation
Supplied as a solution in PBS containing Glycerol and Sodium Azide.

Applications

Recommended Concentration
Sample
Western Blot
Please refer to the PDF datasheet for specifications.

Please Note: Optimal dilutions should be determined by each laboratory for each application.General Protocolsare available in the Technical Information section on our website.

Data Example

Western BlotDetection of Human SUMO1 by Western Blot.View Larger

Detection of Human SUMO1 by Western Blot.Western blot shows lysates of SUMO1. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Human SUMO1 Monoclonal Antibody (Catalog # A-716) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for SUMO1 at approximately 17-19 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: SUMO1

Small Ubiquitin­like Modifiers (SUMOs) are a family of small, related proteins that can be enzymatically attached to a target protein by a post­translational modification process termed SUMOylation. Unlike ubiquitination, which targets proteins for degradation, SUMOylation participates in a number of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. All human SUMO proteins share a conserved ubiquitin-like domain and a C-terminal diglycine cleavage/attachment site. Human SUMO1, also known as Sentrin, UBL1, and SMT3C, is synthesized as a 101 amino acid (aa) propeptide that contains a four aa C­terminal prosegment. Following prosegment cleavage, the C­terminal glycine may be enzymatically attached to a lysine on a target protein. Human SUMO1 shares 100% sequence identity to SUMO1 from mouse. SUMO1 is the most unique of the four identified SUMO proteins and shares only 44%, 47%, and 41% sequence identity to SUMO2, SUMO3, and SUMO4, respectively.

Long Name
Small Ubiquitin-like Modifier 1
Entrez Gene IDs
7341 (Human); 22218 (Mouse); 301442 (Rat)
Alternate Names
DAP1; GAP modifying protein 1; GAP-modifying protein 1; GMP1SMT3CSMT3H3OFC10UBL1PIC1; PIC1; SENP2; Sentrin; small ubiquitin-related modifier 1; SMT3 homolog 3; SMT3 suppressor of mif two 3 homolog 1 (S. cerevisiae); SMT3 suppressor of mif two 3 homolog 1 (yeast); SMT3; SMT3C; SMT3H3; SUMO1; SUMO-1; Ubiquitin-homology domain protein PIC1; ubiquitin-like 1 (sentrin); Ubiquitin-like protein SMT3C; Ubiquitin-like protein UBL1; UBL1

Product Datasheets

Product Datasheet
COA

FAQs

No product specific FAQs exist for this product, however you may

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Isotype Controls

Normal Rat IgG Control(Azide Free)

6-001-F
3Citations
Ctrl

Rat IgG2A Isotype Control

MAB006
108Citations
1Review
1Image
Ctrl

Rat IgG2A Isotype Control

MAB006R
1Image
Ctrl

Normal Rat IgG Control

6-001-A
12Citations
3Reviews
Ctrl

Secondary Antibodies

Goat Anti-Rat IgG Antibody

AF005
2Citations
1Review
WB

Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

NL015
1Citations
Flow,IHC,ICC

Goat Anti-Rat IgG Biotinylated Antibody

BAF005
3Citations
WB

Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

NL014
Flow,IHC,ICC

Goat Anti-Rat F(ab)2 IgG (H+L) APC-conjugated Antibody

F0113
3Citations
1Image
Flow

Rat IgG VisUCyte HRP Polymer Antibody

VC005
1Image
IHC

Rat IgG HRP-conjugated Antibody

HAF005
12Citations
4Reviews
2Images
WB,Simple Western

Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

F0105B
4Citations
1Image
Flow

Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

F0104B
1Review
1Image
Flow

Goat Anti-Rat IgG NorthernLights™ NL557-conjugated Antibody

NL013
3Citations
Flow,IHC,ICC

Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

F0115
1Citations
Flow

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