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CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins.Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration.The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard.In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
| Formulation | Supplied as a solution in 50 mM Hepes pH 7.5, 200 mM NaCl, 10% Glycerol (v/v), 2 mM DTT |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Ubiquitin-conjugating Enzyme Variant 1a (Uev1a), also known as Ubiquitin-conjugating Enzyme E2 Variant 1 (UBE2V1), is a member of the Ubiquitin-conjugating (E2) enzyme family. Human Uev1a/UBE2V1 and its mouse ortholog have predicted molecular weights of 19 kDa and 16.5 kDa, respectively. The human protein shares 89% amino acid (aa) sequence identity with its mouse ortholog. The E2 catalytic core domain of Uev1a/UBE2V1 lacks an active site cysteine residue, rendering it catalytically inactive on its own (1). However, in the cytoplasm Uev1a/UBE2V1 is able to form a catalytically active complex with UBE2N/Ubc13, which mediates the synthesis Lys63-linked Ubiquitin chains and is required for NF-kappa B activation (2,3). Uev1a/UBE2V1 is required for UBE2N (Ubc13)/Uev1a Complex-dependent Lys63-linked Ubiquitin chain formation. More specifically, Uev1a/UBE2V1 orients the Ubiquitin molecule to favor linkage at Lys63 via a non-covalent interaction with the Ubiquitin molecule (4). The human protein contains a non-conserved 30 aa N-terminal tail that functionally differentiates it from Mms2, another E2 enzyme variant and UBE2N/Ubc13 binding partner (4). Pharmacological inhibition of the UBE2N (Ubc13)/Uev1a Complex blocks the proliferation of diffuse large B-cell lymphoma cells, suggesting that this complex may play a role in cancer (5). This protein has an N-terminal His6-tag.
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